将耐热链霉菌11432固定在聚氨酯泡沫上以提高乙酰异戊酰泰乐菌素的产量。

Immobilization of Streptomyces thermotolerans 11432 on polyurethane foam to improve production of acetylisovaleryltylosin.

作者信息

Zhu Hongji, Wang Weihua, Liu Jiaheng, Caiyin Qinggele, Qiao Jianjun

机构信息

Key Laboratory of Systems Bioengineering, Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China.

出版信息

J Ind Microbiol Biotechnol. 2015 Jan;42(1):105-11. doi: 10.1007/s10295-014-1545-x. Epub 2014 Nov 21.

DOI:10.1007/s10295-014-1545-x

PMID:25413211

Abstract

In this study, polyurethane foam (PUF) was chemically treated to immobilize Streptomyces thermotolerans 11432 for semi-continuous production of acetylisovaleryltylosin (AIV). Based on experimental results, positive cross-linked PUF (PCPUF) was selected as the most effective carrier according to immobilized cell mass. The effect of adsorption time on immobilized mass was investigated. AIV concentration (33.54 mg/l) in batch fermentations with immobilized cells was higher than with free cells (20.34 mg/l). In repeated batch fermentations with immobilized S. thermotolerans 11432 using PCPUF cubes, high AIV concentrations and conversion rates were attained, ranging from 25.56 to 34.37 mg/l and 79.93 to 86.31 %, respectively. Significantly, this method provides a feasible strategy for efficient AIV production and offers the potential for large-scale production.

摘要

在本研究中，对聚氨酯泡沫（PUF）进行化学处理，以固定化耐热链霉菌11432用于半连续生产乙酰异戊酰泰乐菌素（AIV）。基于实验结果，根据固定化细胞量，选择正交联聚氨酯泡沫（PCPUF）作为最有效的载体。研究了吸附时间对固定化量的影响。固定化细胞分批发酵中AIV浓度（33.54mg/l）高于游离细胞（20.34mg/l）。使用PCPUF立方体对耐热链霉菌11432进行重复分批发酵，可获得较高的AIV浓度和转化率，分别为25.56至34.37mg/l和79.93至86.31%。值得注意的是，该方法为高效生产AIV提供了一种可行策略，并具有大规模生产的潜力。