Li Miao, Zhang Guangbo, Zhang Xuefeng, Lv Guanglin, Wei Xuedong, Yuan Hexing, Hou Jianquan
Department of Urology, The First Affiliated Hospital of Soochow University, 188 Shizi Road, Suzhou, 215006, People's Republic of China.
Med Oncol. 2014 Dec;31(12):349. doi: 10.1007/s12032-014-0349-1. Epub 2014 Nov 22.
Renal cell carcinoma (RCC) is well known as a typical hypervascular tumor and has a high mortality rate. Tumor-induced angiogenesis is crucial for tumor growth and metastasis. It also plays an important role in the development and progression of RCC. However, the molecular mechanism is still unclear. In our study, we evaluated the expression level of B7-H3 in CD14(+) monocytes in 56 paired RCC samples and distant normal tissues by flow cytometry and located the co-expression of B7-H3 and CD14 by immunohistochemistry. In addition, we analyzed its association with clinical pathologic features through Chi-square test and Fisher exact test. Results showed that B7-H3 and CD14 co-expressed in tumor stroma surrounding the vessels and that the level of B7-H3 expression was higher in tumor than in normal tissues (63.42 ± 11.92 vs. 15.59 ± 3.01, P < 0.0001). Furthermore, the expression level was significantly associated with RCC stage (P = 0.000), nodal metastasis (P = 0.003), distant metastasis (P = 0.020), and nuclear grade (P = 0.004). Conclusively, we found the phenomenon that B7-H3 and CD14 co-expressed in RCC tissues. The level of expression was closely associated with the tumor's progression, indicating that B7-H3 might play an important role in angiogenesis of RCC mediated by CD14(+) monocytes.
肾细胞癌(RCC)是一种典型的富血管肿瘤,死亡率很高。肿瘤诱导的血管生成对肿瘤的生长和转移至关重要。它在肾细胞癌的发生和发展中也起着重要作用。然而,其分子机制仍不清楚。在我们的研究中,我们通过流式细胞术评估了56对肾细胞癌样本和远处正常组织中CD14(+)单核细胞中B7-H3的表达水平,并通过免疫组织化学定位了B7-H3和CD14的共表达。此外,我们通过卡方检验和Fisher精确检验分析了其与临床病理特征的相关性。结果显示,B7-H3和CD14在血管周围的肿瘤基质中共表达,且肿瘤组织中B7-H3的表达水平高于正常组织(63.42±11.92对15.59±3.01,P<0.0001)。此外,表达水平与肾细胞癌分期(P=0.000)、淋巴结转移(P=0.003)、远处转移(P=0.020)和核分级(P=0.004)显著相关。总之,我们发现了肾细胞癌组织中B7-H3和CD14共表达的现象。表达水平与肿瘤进展密切相关,表明B7-H3可能在CD14(+)单核细胞介导的肾细胞癌血管生成中起重要作用。