血小板活化因子对卵磷脂定向多层膜的影响的电子自旋共振监测：血小板活化因子与胆固醇的相互作用

Influence of PAF-acether on lecithin-oriented multibilayers monitored by ESR: interaction of PAF-acether with cholesterol.

作者信息

Olivier J L, Chachaty C, Wolf C, Bereziat G

机构信息

URA CNRS 217, CHU St. Antoine, Paris, France.

出版信息

Biochimie. 1989 Jan;71(1):105-9. doi: 10.1016/0300-9084(89)90139-9.

DOI:10.1016/0300-9084(89)90139-9

PMID:2541796

Abstract

The influence of PAF-acether on natural ovolecithin oriented multibilayers is detected by ESR of intercalated 5 doxyl stearic acid. Simulation of lineshapes demonstrates an enlarged orientational distribution of the local director of the phospholipid phase and a small increase of the order parameter. The amount of PAF-acether required to destabilize the ovolecithin lamellar phase depends on the degree of hydration. By contrast, cholesterol displays an organizing effect on the PAF-acether phase. Simulation establishes a sharp orientational distribution of the local director when cholesterol reaches stoichiometric ratio relative to PAF-acether. At the same time, cholesterol increases the order parameter.

摘要

通过插入的5-硬脂酸二氧磷基胆碱的电子自旋共振（ESR）检测血小板激活因子（PAF-乙醚）对天然卵磷脂定向多层膜的影响。线形模拟表明磷脂相局部指向矢的取向分布扩大，序参量略有增加。使卵磷脂层状相不稳定所需的PAF-乙醚量取决于水合程度。相比之下，胆固醇对PAF-乙醚相具有组织作用。当胆固醇与PAF-乙醚达到化学计量比时，模拟结果显示局部指向矢有明显的取向分布。同时，胆固醇增加了序参量。