ATP酶可能在充血性心力衰竭大鼠能量代谢紊乱中起关键作用。
ATPase may play a critical role in disturbance of energy metabolism in congestive heart failure rats.
作者信息
Song Rong, Zhu Shanjun, Zhang Hongming, Li Xiaoyan, Wu Songlin
机构信息
Department of Intensive Care Unit, The Fifth Hospital of PLA Yinchuan 750004, Ningxia, P. R. China.
Department of Cardiology, Xinqiao Hospital, The Third Military Medical University Chongqing 400038, P. R. China.
出版信息
Int J Clin Exp Med. 2014 Oct 15;7(10):3654-8. eCollection 2014.
OBJECTIVE
This study aims to explore the expression and significance of ATPase in myocardium of congestive heart failure induced by pressure overload in rats.
METHODS
Male SD rats were divided into 3 groups randomly: Control group (C group), coarctation of abdominal aorta 15 weeks group (A group) and sham operation group (SH group). The hemodynamics parameters were measured. The size of adenine acid pool (ATP, ADP) in myocardium was measured by high performance liquid chromatography (HPLC). The mRNA copies of ATPase-α subunit, ATPase-6 subunit in ventricular myocardium were determined by RT-PCR. The protein content of F1-ATPase in mitochondria was detected by Western blot analysis.
RESULTS
The ±dp/dtmax and contents of ATP and ADP decreased significantly in myocardium were decreased in A group. Compared with C and SH group, the mRNA copies of ATPase-α subunit, ATPase-6 subunit and the protein content of F1-ATPase were decreased in A group.
CONCLUSION
The expression of F1-ATPase decreased parallel with abnormal of the adenine nucleotide in myocardium of congestive heart failure. It may play a critical role in disturbance of energy metabolism in disfunction of heart.
目的
本研究旨在探讨三磷酸腺苷酶（ATPase）在压力超负荷诱导的大鼠充血性心力衰竭心肌中的表达及意义。
方法
雄性SD大鼠随机分为3组：对照组（C组）、腹主动脉缩窄15周组（A组）和假手术组（SH组）。测量血流动力学参数。采用高效液相色谱法（HPLC）测定心肌中腺嘌呤酸池（ATP、ADP）的大小。通过逆转录聚合酶链反应（RT-PCR）测定心室肌中ATPase-α亚基、ATPase-6亚基的mRNA拷贝数。采用蛋白质免疫印迹法检测线粒体中F1-ATPase的蛋白含量。
结果
A组心肌的±dp/dtmax以及ATP和ADP含量显著降低。与C组和SH组相比，A组ATPase-α亚基、ATPase-6亚基的mRNA拷贝数以及F1-ATPase的蛋白含量降低。
结论
充血性心力衰竭心肌中F1-ATPase的表达与腺嘌呤核苷酸异常平行降低。它可能在心脏功能障碍时能量代谢紊乱中起关键作用。
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