使用来自有或无生殖器人乳头瘤病毒（HPV）诱发病变的HIV阴性女性的肛门内标本进行液基细胞学检查和HPV DNA检测。

Liquid-based cytology and HPV DNA testing using intra-anal specimens from HIV-negative women with and without genital HPV-induced lesions.

作者信息

Eleutério José, Benício Givanildo Carneiro, Giraldo Paulo César, Gonçalves Ana Katherine Silveira, Eleutério Renata Mírian Nunes, Oliveira Denise Nunes, Jacyntho Cláudia

机构信息

Department of Maternal and Child Health, Faculty of Medicine, Federal University of Ceará, Fortaleza, Brazil; Department of Pathology, Faculty of Medicine, Federal University of Ceará, Fortaleza, Brazil.

出版信息

Diagn Cytopathol. 2015 May;43(5):360-5. doi: 10.1002/dc.23238. Epub 2014 Nov 24.

DOI:10.1002/dc.23238

PMID:25421882

Abstract

BACKGROUND

Screening for anal cancer using cytology has not been considered in immunocompetent women. The aim of this study was to identify cytological atypia and human papillomavirus (HPV) DNA in intra-anal specimens from human immunodeficiency virus (HIV)-negative women with and without genital HPV lesions.

METHODS

This study was a cross-sectional analysis of 142 women who were negative for the HIV: 80 with genital lesions that were associated with HPV and 62 without HPV-induced lesions. The women were evaluated at the Federal University of Ceará from October 2011 to June 2012. The statistical analysis included the Fisher exact test and the odds ratio (CI 95%).

RESULTS

Atypical anal cytology was observed in 24 (29.3%) patients in the study group and in 11 (17.8%) patients in the control group. In cases with at least two sites of HPV-associated lesions, 12 (41.4%) presented atypical cytology (P = 0.0220; OR = 2.7621, 1.1579-6.5889). When the practice of anal sex was evaluated, atypical cytology was observed in 22/43 (34%) [P = 0.0214; OR = 2.519, 1.146-5.534]. HPV DNA was detected in 17/27 (63%) cases with at least two sites of lesions (P = 0.0293, OR = 2.4855, 1.0960-5.6367). In the 33 cases who presented positive HPV DNA test results, the liquid-based cytology results were atypical (P = 0.0212, OR = 2.8, 1.1665-6.7208).

CONCLUSION

Based on the results, liquid-based cytology may be used to detect intra-anal lesions, especially among women who have a history of anal intercourse or who have genital HPV-associated lesions at multiple sites.

摘要

背景

免疫功能正常的女性未被纳入采用细胞学方法筛查肛门癌的研究对象。本研究旨在确定感染人类免疫缺陷病毒（HIV）阴性且有或无生殖器人乳头瘤病毒（HPV）病变的女性肛门内标本中的细胞学异型性和HPV DNA。

方法

本研究对142名HIV阴性女性进行横断面分析：80名有与HPV相关的生殖器病变，62名无HPV诱发的病变。2011年10月至2012年6月在塞阿拉联邦大学对这些女性进行评估。统计分析包括Fisher精确检验和比值比（95%置信区间）。

结果

研究组24例（29.3%）患者及对照组11例（17.8%）患者观察到非典型肛门细胞学表现。在至少有两个HPV相关病变部位的病例中，12例（41.4%）出现非典型细胞学表现（P = 0.0220；比值比 = 2.7621，1.1579 - 6.5889）。评估肛交行为时，22/43例（34%）观察到非典型细胞学表现[P = 0.0214；比值比 = 2.519，1.146 - 5.534]。在至少有两个病变部位的17/27例（63%）病例中检测到HPV DNA（P = 0.0293，比值比 = 2.4855，1.0960 - 5.6367）。在33例HPV DNA检测结果呈阳性的病例中，液基细胞学结果为非典型（P = 0.0212，比值比 = 2.8，1.1665 - 6.7208）。