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从桑枝中制备桑枝酮及其对移植性 H22 肝癌小鼠的作用。

Preparation of morusin from Ramulus mori and its effects on mice with transplanted H22 hepatocarcinoma.

机构信息

Silk Biotechnology Laboratory, School of Biology and Basic Medical Sciences, Soochow University, Suzhou, 215123, People's Republic of China.

出版信息

Biofactors. 2014 Nov-Dec;40(6):636-45. doi: 10.1002/biof.1191. Epub 2014 Nov 25.

Abstract

In this study, we used branches Ramulus mori from cultivated mulberry Husang-32 (Morus multicaulis Perry) as the experimental material and anhydrous ethanol as the extraction solution to obtain the crude extract from the branch bark. The ethanolic extract was successively purified through a macroporous resin, Sephadex LH-20, and semipreparative reversed-phase high-performance liquid chromatography (RP-HPLC). The high-purity monomer was identified as morusin by HPLC with diode array detection (HPLC-DAD) and its UV spectrum. The contents of morusin exhibited almost no difference between the root and branch bark in Husang-32, and morusin was not detected in the leaves. Morusin is able to inhibit the tumor growth of transplanted H22 hepatocarcinoma in mice and has no side effects. The fluorescence quantitative real-time PCR (qRT-PCR) results indicate that morusin has a marked inhibitory effect on liver cancer cells through a mechanism that may be related increases in the expression of p53, Survivin, CyclinB1, and Caspase-3 and a decrease in NF-κ B gene expression. The influence of this compound is more apparent in the Caspase-3 and the NF-κ B genes.

摘要

在这项研究中,我们使用了家桑(Morus multicaulis Perry)的桑枝作为实验材料,以无水乙醇作为提取溶液,从桑枝皮中获得粗提物。醇提物依次经大孔树脂、葡聚糖凝胶 LH-20 和半制备反相高效液相色谱(RP-HPLC)进行纯化。用二极管阵列检测高效液相色谱(HPLC-DAD)及其紫外光谱法鉴定高纯度单体为桑辛素。在 Husang-32 中,桑辛素在根皮和桑枝皮中的含量几乎没有差异,而在桑叶中则未检测到桑辛素。桑辛素能够抑制荷瘤小鼠移植性 H22 肝癌的生长,且无副作用。荧光定量实时 PCR(qRT-PCR)结果表明,桑辛素可能通过增加 p53、Survivin、CyclinB1 和 Caspase-3 的表达以及降低 NF-κB 基因表达来抑制肝癌细胞,该化合物的影响在 Caspase-3 和 NF-κB 基因中更为明显。

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