Wolkers Willem F, Oldenhof Harriëtte
Institute of Multiphase Processes, Leibniz Universität Hannover, Callinstrasse 36, D-30167, Hannover, Germany,
Methods Mol Biol. 2015;1257:147-61. doi: 10.1007/978-1-4939-2193-5_5.
An infrared spectrum gives information about characteristic molecular vibrations of specific groups in molecules. Fourier transform infrared spectroscopy can be applied to study lipids and proteins in cells or tissues. Spectra can be collected during cooling, heating, or dehydration of a sample using a temperature-controlled sample holder or a sample holder for controlled dehydration. In the current chapter, acquisition and analysis of infrared spectra during cooling, warming, or dehydration is described. Spectra analysis involving assessment of specific band positions, areas, or ratios is described. Special emphasis is given on studying membrane phase behavior and protein denaturation in cells or tissues. In addition, methods are presented to determine the water-to-ice phase change during freezing, dehydration kinetics, and the glass transition temperature of amorphous systems.
红外光谱提供有关分子中特定基团特征分子振动的信息。傅里叶变换红外光谱可用于研究细胞或组织中的脂质和蛋白质。使用控温样品架或用于控制脱水的样品架，可以在样品冷却、加热或脱水过程中收集光谱。在本章中，将描述在冷却、升温或脱水过程中红外光谱的采集和分析。还将描述涉及评估特定谱带位置、面积或比率的光谱分析。特别强调研究细胞或组织中的膜相行为和蛋白质变性。此外，还介绍了确定冷冻过程中水到冰的相变、脱水动力学以及非晶系统玻璃化转变温度的方法。
