Fleck Roland A
Centre for Ultrastructural Imaging, King's College London, New Hunts House, Guy's Campus, London, SE1 1UL, UK,
Methods Mol Biol. 2015;1257:243-74. doi: 10.1007/978-1-4939-2193-5_9.
Low-temperature electron microscopy endeavors to provide "solidification of a biological specimen by cooling with the aim of minimal displacement of its components through the use of low temperature as a physical fixation strategy" (Steinbrecht and Zierold, Cryotechniques in biological electron microscopy. Springer-Verlag, Berlin, p 293, 1987). The intention is to maintain confidence that the tissue observed retains the morphology and dimensions of the living material while also ensuring soluble cellular components are not displaced. As applied to both scanning and transmission electron microscopy, cryo-electron microscopy is a strategy whereby the application of low-temperature techniques are used to reduce or remove processing artifacts which are commonly encountered in more conventional room temperature electron microscopy techniques which rely heavily on chemical fixation and heavy metal staining. Often, cryo-electron microscopy allows direct observation of specimens, which have not been stained or chemically fixed.
低温电子显微镜旨在通过冷却实现“生物样本的固化，目的是利用低温作为一种物理固定策略，使样本成分的位移最小化”（施泰因布雷希特和齐罗尔德，《生物电子显微镜中的低温技术》。施普林格出版社，柏林，第293页，1987年）。其目的是让人相信所观察的组织保留了活体材料的形态和尺寸，同时确保可溶性细胞成分不会发生位移。低温电子显微镜应用于扫描电子显微镜和透射电子显微镜，它是一种策略，即运用低温技术来减少或消除在更传统的室温电子显微镜技术中常见的处理伪像，传统室温电子显微镜技术严重依赖化学固定和重金属染色。通常，低温电子显微镜允许直接观察未染色或未进行化学固定的样本。
