• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Determination of the steady-state turnover rates of the metabolically active pools of phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate in human erythrocytes.人红细胞中磷酸肌醇4-磷酸和磷酸肌醇4,5-二磷酸代谢活性池稳态周转率的测定
Biochem J. 1989 May 1;259(3):893-6. doi: 10.1042/bj2590893.
2
Turnover of phosphomonoester groups and compartmentation of polyphosphoinositides in human erythrocytes.人红细胞中磷酸单酯基团的周转和多磷酸肌醇的区室化
Biochem J. 1986 May 1;235(3):775-83. doi: 10.1042/bj2350775.
3
Turnover of the phosphomonoester groups of polyphosphoinositol lipids in unstimulated human platelets.未受刺激的人血小板中多磷酸肌醇脂质磷酸单酯基团的周转
Eur J Biochem. 1987 Jul 1;166(1):3-9. doi: 10.1111/j.1432-1033.1987.tb13475.x.
4
Rapid breakdown of phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate in rat hepatocytes stimulated by vasopressin and other Ca2+-mobilizing hormones.血管加压素和其他可动员钙离子的激素刺激下大鼠肝细胞中磷脂酰肌醇4-磷酸和磷脂酰肌醇4,5-二磷酸的快速分解。
Biochem J. 1983 Jun 15;212(3):733-47. doi: 10.1042/bj2120733.
5
Phosphoinositide hydrolysis by guanosine 5'-[gamma-thio]triphosphate-activated phospholipase C of turkey erythrocyte membranes.火鸡红细胞膜中由鸟苷5'-[γ-硫代]三磷酸激活的磷脂酶C引起的磷酸肌醇水解作用。
Biochem J. 1988 Jun 1;252(2):583-93. doi: 10.1042/bj2520583.
6
Antigen receptor-mediated regulation of sustained polyphosphoinositide turnover in a human T cell line. Evidence for a receptor-regulated pathway for production of phosphatidylinositol 4,5-bisphosphate.抗原受体介导的人T细胞系中持续多磷酸肌醇周转的调节。磷脂酰肌醇4,5-二磷酸产生的受体调节途径的证据。
J Biol Chem. 1990 Apr 15;265(11):5983-9.
7
Pathway of synthesis of 3,4- and 4,5-phosphorylated phosphatidylinositols in the duckweed Spirodela polyrhiza L.浮萍（紫萍）中3,4-和4,5-磷酸化磷脂酰肌醇的合成途径
Biochem J. 1993 Feb 15;290 ( Pt 1)(Pt 1):145-50. doi: 10.1042/bj2900145.
8
Differential regulation by phosphatidylinositol 4,5-bisphosphate of pituitary plasma-membrane and cytosolic phosphoinositide kinases.垂体质膜和胞质磷酸肌醇激酶受磷脂酰肌醇4,5-二磷酸的差异调节。
Biochem J. 1986 Dec 1;240(2):341-8. doi: 10.1042/bj2400341.
9
Phosphatidylinositol 4,5-bisphosphate turnover is transient while phosphatidylinositol turnover is persistent in thyrotropin-releasing hormone-stimulated rat pituitary cells.在促甲状腺激素释放激素刺激的大鼠垂体细胞中，磷脂酰肌醇4,5-二磷酸的周转是短暂的，而磷脂酰肌醇的周转是持续的。
Proc Natl Acad Sci U S A. 1986 Nov;83(22):8540-4. doi: 10.1073/pnas.83.22.8540.
10
Multiple metabolic pools of phosphoinositides and phosphatidate in human erythrocytes incubated in a medium that permits rapid transmembrane exchange of phosphate.在允许磷酸盐快速跨膜交换的培养基中孵育的人红细胞中磷酸肌醇和磷脂酸的多个代谢池。
Biochem J. 1987 May 15;244(1):209-17. doi: 10.1042/bj2440209.

引用本文的文献

1
Acyl chain selection couples the consumption and synthesis of phosphoinositides.酰基链选择将磷酸肌醇的消耗和合成偶联在一起。
EMBO J. 2022 Sep 15;41(18):e110038. doi: 10.15252/embj.2021110038. Epub 2022 Jun 30.
2
Crucial Players for Inter-Organelle Communication: PI5P4Ks and Their Lipid Product PI-4,5-P Come to the Surface.细胞器间通讯的关键参与者：PI5P4K 及其脂质产物 PI-4,5-P 浮出水面。
Front Cell Dev Biol. 2022 Jan 7;9:791758. doi: 10.3389/fcell.2021.791758. eCollection 2021.
3
Control of diverse subcellular processes by a single multi-functional lipid phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2].单一多功能脂质磷脂酰肌醇4,5-二磷酸[PI(4,5)P2]对多种亚细胞过程的调控。
Biochem J. 2016 Jun 15;473(12):1681-92. doi: 10.1042/BCJ20160069.
4
Regulation of platelet plug formation by phosphoinositide metabolism.磷酸肌醇代谢调节血小板栓形成。
Blood. 2013 Aug 22;122(8):1358-65. doi: 10.1182/blood-2013-05-427716. Epub 2013 Jun 11.
5
Activation of phosphatidylinositol 4,5-bisphosphate supply by agonists and non-hydrolysable GTP analogues.激动剂和不可水解的GTP类似物对磷脂酰肌醇4,5-二磷酸供应的激活作用。
Biochem J. 1993 Dec 1;296 ( Pt 2)(Pt 2):481-8. doi: 10.1042/bj2960481.
6
Evidence for substrate-cycling of 3-, 3,4-, 4-, and 4,5-phosphorylated phosphatidylinositols in plants.植物中3-、3,4-、4-和4,5-磷酸化磷脂酰肌醇底物循环的证据。
Biochem J. 1995 Nov 1;311 ( Pt 3)(Pt 3):1001-7. doi: 10.1042/bj3111001.

本文引用的文献

1
Inositol lipids and membrane function in erythrocytes.红细胞中的肌醇脂质与膜功能
Cell Calcium. 1982 Oct;3(4-5):451-65. doi: 10.1016/0143-4160(82)90030-6.
2
ATP utilizing reactions of human erythrocyte membranes and the influence of modulator proteins.人红细胞膜的ATP利用反应及调节蛋白的影响
Acta Biol Med Ger. 1981;40(4-5):479-86.
3
Turnover of phosphomonoester groups and compartmentation of polyphosphoinositides in human erythrocytes.人红细胞中磷酸单酯基团的周转和多磷酸肌醇的区室化
Biochem J. 1986 May 1;235(3):775-83. doi: 10.1042/bj2350775.
4
Quantitation of adenosine-5'-triphosphate used for phosphoinositide metabolism in human erythrocytes.用于人红细胞中磷酸肌醇代谢的三磷酸腺苷（ATP）的定量分析。
Blood. 1985 Nov;66(5):1133-7.
5
Multiple metabolic pools of phosphoinositides and phosphatidate in human erythrocytes incubated in a medium that permits rapid transmembrane exchange of phosphate.在允许磷酸盐快速跨膜交换的培养基中孵育的人红细胞中磷酸肌醇和磷脂酸的多个代谢池。
Biochem J. 1987 May 15;244(1):209-17. doi: 10.1042/bj2440209.
6
Human erythrocyte 2,3-diphosphoglycerate metabolism. Influence of 1,3-diphosphoglycerate and Pi. In vitro studies at low pH with computer simulations.人红细胞2,3-二磷酸甘油酸代谢。1,3-二磷酸甘油酸和无机磷酸的影响。低pH条件下的体外研究及计算机模拟
Arch Biochem Biophys. 1978 Sep;190(1):67-84. doi: 10.1016/0003-9861(78)90254-0.

人红细胞中磷酸肌醇4-磷酸和磷酸肌醇4,5-二磷酸代谢活性池稳态周转率的测定

Determination of the steady-state turnover rates of the metabolically active pools of phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate in human erythrocytes.

作者信息

King C E, Hawkins P T, Stephens L R, Michell R H

机构信息

Department of Biochemistry, University of Birmingham, U.K.

出版信息

Biochem J. 1989 May 1;259(3):893-6. doi: 10.1042/bj2590893.

DOI:10.1042/bj2590893
PMID:2543372
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1138600/
Abstract

When intact human erythrocytes are incubated at metabolic steady state in a chloride-free medium containing [32P]Pi, there is rapid labelling of the gamma-phosphate of ATP, followed by a slower labelling of the monoester phosphate groups of phosphatidylinositol 4-phosphate (PtdIns4P) and phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P2] [King, Stephens, Hawkins, Guy & Michell (1987) Biochem. J. 244, 209-217]. We have analysed the early kinetics of the labelling of these phosphate groups, in order to determine: (a) the steady-state rates of the interconversions of phosphatidylinositol, PtdIns4P and PtdIns(4,5)P2; and (b) the fractions of the total cellular complement of PtdIns4P and PtdIns(4,5)P2 that participate in this steady-state turnover. The experimental data most closely fit a pattern of PtdIns4P and PtdIns(4,5)P2 turnover in which one-quarter of the total cellular complement of each lipid is in the metabolic pool that participates in rapid metabolic turnover, with rate constants of 0.028 min-1 for the interconversion of PtdIns and PtdIns4P, and of 0.010 min-1 for the PtdIns4P/PtdIns(4,5)P2 cycle. These rate constants represent metabolic fluxes of approx. 2.1 nmol of lipid/h per ml of packed erythrocytes between PtdIns and PtdIns4P and of approx. 5.7 nmol/h per ml of cells between PtdIns4P and PtdIns(4,5)P2.

摘要

当完整的人体红细胞在含有[32P]Pi的无氯培养基中于代谢稳态下孵育时，ATP的γ-磷酸会迅速被标记，随后磷脂酰肌醇4-磷酸（PtdIns4P）和磷脂酰肌醇4,5-二磷酸[PtdIns(4,5)P2]的单酯磷酸基团被标记的速度较慢[金、斯蒂芬斯、霍金斯、盖伊和米切尔（1987年）《生物化学杂志》244卷，209 - 217页]。我们分析了这些磷酸基团标记的早期动力学，以便确定：（a）磷脂酰肌醇、PtdIns4P和PtdIns(4,5)P2相互转化的稳态速率；以及（b）参与这种稳态周转的PtdIns4P和PtdIns(4,5)P2在细胞总含量中所占的比例。实验数据最符合PtdIns4P和PtdIns(4,5)P2周转的一种模式，即每种脂质细胞总含量的四分之一处于参与快速代谢周转的代谢池中，PtdIns和PtdIns4P相互转化的速率常数为0.028 min-1，PtdIns4P/PtdIns(4,5)P2循环的速率常数为0.010 min-1。这些速率常数代表了PtdIns和PtdIns4P之间约2.1 nmol脂质/（h·ml压积红细胞）以及PtdIns4P和PtdIns(4,5)P2之间约5.7 nmol/（h·ml细胞）的代谢通量。