Bai Li, Hu Yujie, Wang Jiahui, Wu Qing, He Yingying, Wang Wei, Gan Xin, Han Chunhui, Li Fengqin, Xu Jin
Wei Sheng Yan Jiu. 2014 Sep;43(5):797-804.
To evaluate the suitability of stx-PCR, Vero cell assay and commercial enzyme immunoassay for detection of Shiga toxin Escherichia coli and to compare sensitivity and specificity of three different methods for detection of Shiga toxin-producing Escherichia coli.
Using stx-PCR, Vero cell assay and commercial enzyme immunoassay to detect 35 Escherichia coli reference strains and 45 strains isolated from food.
The three methods all had good specificity. 31 strains gave positive reaction in the Vero cell assay and in the stx-PCR. The consistency between the Vero cell assay and stx-PCR was 100%. Only 38 strains can be detected by commercial enzyme immunoassay.
stx-PCR method can serve as a routine rapid detection method in the laboratory. Vero cell assay is recommended to be the gold standard to determine whether the bacteria had the functionally active toxin. Commercial kit was suitable for preliminary rapid detection during clinical testing and outbreaks of food-borne disease.
评估stx - PCR、Vero细胞检测法和商业酶免疫测定法检测志贺毒素大肠杆菌的适用性，并比较这三种不同方法检测产志贺毒素大肠杆菌的敏感性和特异性。
采用stx - PCR、Vero细胞检测法和商业酶免疫测定法检测35株大肠杆菌参考菌株和45株从食品中分离出的菌株。
三种方法均具有良好的特异性。31株菌株在Vero细胞检测法和stx - PCR中呈阳性反应。Vero细胞检测法和stx - PCR之间的一致性为100%。商业酶免疫测定法仅能检测出38株菌株。
stx - PCR方法可作为实验室常规快速检测方法。建议将Vero细胞检测法作为确定细菌是否具有功能活性毒素的金标准。商业试剂盒适用于临床检测和食源性疾病暴发期间的初步快速检测。
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