Equivalence between the equilibrium dispersive and the transport model in describing band broadening in analytical gradient liquid chromatography.
作者信息
Hao Weiqiang, Di Bin, Yue Bangyi, Chen Qiang, Wu Shun, Zhang Peipei
机构信息
High-Tech Research Institute of Nanjing University, Changzhou 213164, China; Key Laboratory of Cigarette Smoke Research, CNTC, Shanghai Tobacco Group Co. LTD, Shanghai 200082, China.
Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing 210009, China.
出版信息
J Chromatogr A. 2014 Nov 21;1369:191-5. doi: 10.1016/j.chroma.2014.10.015. Epub 2014 Oct 19.
It is shown that the transport (TR) model may be equivalent to the equilibrium dispersive (ED) model in analytical gradient liquid chromatography when the column efficiency is not small. This result is obtained by assuming that the gradient profile is not distorted, the injection is an impulse, and the apparent axial dispersion coefficient (Da) of the ED model or the lumped kinetic coefficient (kfL) of the TR model varies with mobile phase composition. According to this equivalence, the plate height equation derived from the TR model in our previous work (Eq. (91) in Hao et al., 2013) is also suited for the ED model. This conclusion is confirmed by comparing the equation with the results obtained by Gritti and Guiochon from the ED model (Gritti and Guiochon, 2012).
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