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紫外线辐射(UVA+UVB)和铜对红藻细基江蓠的形态、超微结构组织及生理反应的影响

Effects of ultraviolet radiation (UVA+UVB) and copper on the morphology, ultrastructural organization and physiological responses of the red alga Pterocladiella capillacea.

作者信息

Schmidt Éder C, Kreusch Marianne, Felix Marthiellen R de L, Pereira Debora T, Costa Giulia B, Simioni Carmen, Ouriques Luciane C, Farias-Soares Francine L, Steiner Neusa, Chow Fungyi, Ramlov Fernanda, Maraschin Marcelo, Bouzon Zenilda L

机构信息

Postdoctoral Research of Postgraduate Program in Cell Biology and Development, Department of Cell Biology, Embryology and Genetics, Federal University of Santa Catarina, Florianópolis, SC, Brazil; Department of Cell Biology, Embryology and Genetics, Plant Cell Biology Laboratory, Federal University of Santa Catarina, Florianópolis, SC, Brazil.

出版信息

Photochem Photobiol. 2015 Mar-Apr;91(2):359-70. doi: 10.1111/php.12396. Epub 2015 Jan 8.

Abstract

The effect of ultraviolet (UV) radiation and copper (Cu) on apical segments of Pterocladiella capillacea was examined under two different conditions of radiation, PAR (control) and PAR+UVA+UVB (PAR+UVAB), and three copper concentrations, ranging from 0 (control) to 0.62, 1.25 and 2.50 μm. Algae were exposed in vitro to photosynthetically active radiation (PAR) at 70 μmol photons m(-2)  s(-1) , PAR + UVB at 0.35 W m(-2) and PAR +UVA at 0.70 W m(-2) during a 12-h photocycle for 3 h each day for 7 days. The effects of radiation and copper on growth rates, content of photosynthetic pigments and photosynthetic performance were analyzed. In addition, samples were processed for light and transmission electron microscopy. The content of photosynthetic pigments decreased after exposure to radiation and Cu. Compared with PAR radiation and copper treatments modified the kinetics patterns of the photosynthesis/irradiance curve. The treatments also caused changes in the ultrastructure of cortical and subcortical cells, including increased cell wall thickness and accumulation of plastoglobuli, as well as changes in the organization of chloroplasts. The results indicate that the synergistic interaction between UV radiation and Cu in P. capillacea, led to the failure of protective mechanisms and causing more drastic changes and cellular imbalances.

摘要

在两种不同的辐射条件下,即光合有效辐射(PAR,对照)和PAR + UVA + UVB(PAR + UVAB),以及三种铜浓度(范围从0(对照)到0.62、1.25和2.50μm)下,研究了紫外线(UV)辐射和铜(Cu)对细基江蓠繁枝变种顶端片段的影响。藻类在体外每天接受12小时的光循环,其中光合有效辐射(PAR)为70μmol光子·m⁻²·s⁻¹,PAR + UVB为0.35 W·m⁻²,PAR + UVA为0.70 W·m⁻²,每种辐射处理3小时,持续7天。分析了辐射和铜对生长速率、光合色素含量和光合性能的影响。此外,对样品进行了光学和透射电子显微镜处理。暴露于辐射和铜后,光合色素含量下降。与PAR辐射相比,铜处理改变了光合作用/辐照度曲线的动力学模式。这些处理还导致皮层和皮层下细胞的超微结构发生变化,包括细胞壁厚度增加和质体小球积累以及叶绿体组织变化。结果表明,UV辐射和Cu在细基江蓠繁枝变种中的协同相互作用导致保护机制失效,并引起更剧烈的变化和细胞失衡。

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