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呼吸道合胞病毒转化的大鼠(GCA、W12和XC)细胞的非贴壁依赖性生长。

Anchorage-independent growth of RSV-transformed rat (GCA, W 12 and XC) cells.

作者信息

Klein A, Kosz-Vnenchak M, Madeja Z, Szuster A

机构信息

Institute of Molecular Biology, Jagellonian University, Kraków, Poland.

出版信息

Folia Histochem Cytobiol. 1989;27(1):11-7. doi: 10.1007/BF02695445.

Abstract

Three RSV-transformed rat cell lines: GCA, W12 and XC were characterized as to their ability to anchorage-independent growth in comparison to normal rat kidney (NRK-49F) cells. Differences in the threshold density (TD) and colony forming efficiency (CFE) of the investigated cells are described. The ability of virally transformed cells to stimulation of soft agar colony formation of NRK cells in coculture assay was presented. The production of TGFs-like factors by GCA, W12 and XC cells was suggested.

摘要

三种经呼吸道合胞病毒(RSV)转化的大鼠细胞系:GCA、W12和XC,与正常大鼠肾(NRK - 49F)细胞相比,对其不依赖贴壁生长的能力进行了表征。描述了所研究细胞在阈值密度(TD)和集落形成效率(CFE)方面的差异。展示了病毒转化细胞在共培养试验中刺激NRK细胞软琼脂集落形成的能力。提出了GCA、W12和XC细胞产生类转化生长因子(TGFs)的情况。

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