van Leeuwen Sander S, Kuipers Bas J H, Dijkhuizen Lubbert, Kamerling Johannis P
Microbial Physiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Nijenborgh 7, NL-9747 AG Groningen, The Netherlands.
FrieslandCampina, Stationsplein 4, NL-3818 LE Amersfoort, The Netherlands.
Carbohydr Res. 2014 Dec 5;400:59-73. doi: 10.1016/j.carres.2014.08.012. Epub 2014 Sep 1.
Vivinal® GOS is a galacto-oligosaccharide (GOS) product, prepared from lactose by incubation with Bacillus circulans β-galactosidase (EC 3.2.1.23). This complex mixture of saccharides with degree of polymerization (DP) between 1 and 8 is generally applied in infant nutrition. Here, a detailed structural description of the commercial product up to the DP5 level is given. First, Vivinal® GOS was subjected to DP analysis using HPLC-SEC (Rezex RSO-01 oligosaccharide Ag(+) column) and (1)H NMR analysis. Then, the product was fractionated on Bio-Gel P-2, and the obtained fractions were pooled according to DP, as indicated by MALDI-TOF-MS analysis. Finally, fractions of single DP, as well as their subfractions obtained by HPAEC-PAD on CarboPac PA-1, were analyzed by 1D/2D (1)H/(13)C NMR spectroscopy and linkage analysis. In total, over 40 structures, providing a structural coverage of over 99% of the product, have been characterized. Detailed (1)H and (13)C NMR data, as well as G.U. values (glucose units; malto-oligosaccharide ladder) on CarboPac PA-1 of all oligosaccharides are included.
Vivinal® GOS是一种低聚半乳糖（GOS）产品，由乳糖与环状芽孢杆菌β-半乳糖苷酶（EC 3.2.1.23）孵育制备而成。这种聚合度（DP）在1至8之间的糖类复杂混合物通常应用于婴儿营养领域。在此，给出了该商业产品直至DP5水平的详细结构描述。首先，使用高效液相色谱-尺寸排阻色谱法（HPLC-SEC，Rezex RSO-01寡糖银（+）柱）和核磁共振氢谱（1H NMR）分析对Vivinal® GOS进行DP分析。然后，将该产品在Bio-Gel P-2上进行分级分离，并根据基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）分析所示的DP对获得的级分进行合并。最后，通过一维/二维核磁共振氢谱/核磁共振碳谱（1D/2D 1H/13C NMR）光谱和键合分析对单-DP级分及其通过CarboPac PA-1上的高效阴离子交换色谱-脉冲安培检测法（HPAEC-PAD）获得的亚级分进行分析。总共已鉴定出40多种结构，其结构覆盖率超过该产品的99%。文中包含了所有寡糖在CarboPac PA-1上的详细核磁共振氢谱和核磁共振碳谱数据以及葡萄糖单位值（G.U.值；麦芽寡糖阶梯）。
