Wadsö Lars, Hansen Lee D
Building Materials, Lund University, Box 118, 221 00 Lund, Sweden.
Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USA.
Methods. 2015 Apr;76:11-19. doi: 10.1016/j.ymeth.2014.10.024. Epub 2014 Oct 28.
Calorespirometry is the simultaneous measurement of heat and gas exchange from biological systems. Such measurements can be used to assess fundamental properties of many different types of systems from small ecosystems to isolated tissues. Techniques for calorespirometric measurements on terrestrial (non-aquatic) samples are described. Methods and models for evaluation of carbon conversion efficiencies, growth rates, and responses to environmental variables from calorespirometric measurements are described. A realistic model of the system under study is essential in the evaluation. Calorespirometry allows testing of models for tissues, individual organisms, and ecosystems.
热呼吸测定法是对生物系统的热量和气体交换进行同步测量。此类测量可用于评估从小型生态系统到离体组织等许多不同类型系统的基本特性。本文描述了对陆地(非水生)样本进行热呼吸测定的技术。阐述了根据热呼吸测定评估碳转化效率、生长速率以及对环境变量响应的方法和模型。在所研究系统的评估中,一个逼真的模型至关重要。热呼吸测定法可用于测试针对组织、个体生物和生态系统的模型。