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转化生长因子-βⅡ型受体及DPC4/Smad4在非小细胞肺癌中的表达及意义

Expression and significance of transforming growth factor-β receptor type II and DPC4/Smad4 in non-small cell lung cancer.

作者信息

Chen Hong, Wang Jing-Wei, Liu Li-Xin, Yan Ji-Dong, Ren Shu-Hua, Li Yan, Lu Zheng

机构信息

Department of Radiotherapy and Chemotherapy, Tangshan Gongren Hospital, Tangshan, Hebei 063000, P.R. China.

Department of Thoracic Surgery, Tangshan Gongren Hospital, Tangshan, Hebei 063000, P.R. China.

出版信息

Exp Ther Med. 2015 Jan;9(1):227-231. doi: 10.3892/etm.2014.2065. Epub 2014 Nov 12.

DOI:10.3892/etm.2014.2065
PMID:25452807
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4247285/
Abstract

The aim of the present study was to investigate the expression levels of transforming growth factor-β (TGF-β) receptor type II (TβRII) and DPC4/Smad4 in the TGF-β signaling pathway and the importance of these expression levels in non-small cell lung cancer (NSCLC). The mRNA and protein expression levels of TβRII and DPC4/Smad4 were detected by reverse transcription-quantitative polymerase chain reaction and western blotting, respectively, in NSCLC and control nonlesional lung tissues of 60 patients. The protein expression levels of DPC4/Smad4 were detected by immunohistochemistry in paraffin-embedded samples of NSCLC. In addition, the correlations among the expression levels of TβRII and DPC4/Smad4 and their association with the clinical and pathological features of NSCLC were analyzed. The expression levels of TβRII and DPC4/Smad4 in NSCLC tissues were significantly lower when compared with the control nonlesional lung tissues (P<0.05). In addition, the expression of TβRII and DPC4/Smad4 in poorly-differentiated NSCLC tissues was significantly lower compared with moderately- or well-differentiated NSCLC tissues (P<0.05). The expression levels of TβRII and DPC4/Smad4 were significantly lower in NSCLC tissues with metastatic lymph nodes compared with tissue without metastatic lymph nodes (P<0.05). Thus, the expression levels were demonstrated to significantly correlate with the clinical and pathological stages, and subsequently were shown to be associated with the occurrence and progression of NSCLC. In conclusion, TβRII and DPC4/Smad4 may play an important role in the tumorigenesis, differentiation and progression of NSCLC via the TGF-β signaling pathway.

摘要

本研究的目的是调查转化生长因子-β（TGF-β）信号通路中II型TGF-β受体（TβRII）和DPC4/Smad4的表达水平，以及这些表达水平在非小细胞肺癌（NSCLC）中的重要性。分别采用逆转录定量聚合酶链反应和蛋白质印迹法检测了60例患者NSCLC组织及对照非病变肺组织中TβRII和DPC4/Smad4的mRNA和蛋白质表达水平。通过免疫组织化学法检测了NSCLC石蜡包埋样本中DPC4/Smad4的蛋白质表达水平。此外，分析了TβRII和DPC4/Smad4表达水平之间的相关性及其与NSCLC临床和病理特征的关系。与对照非病变肺组织相比，NSCLC组织中TβRII和DPC4/Smad4的表达水平显著降低（P<0.05）。此外，与中分化或高分化NSCLC组织相比，低分化NSCLC组织中TβRII和DPC4/Smad4的表达显著降低（P<0.05）。与无转移淋巴结的组织相比，有转移淋巴结的NSCLC组织中TβRII和DPC4/Smad4的表达水平显著降低（P<0.05）。因此，这些表达水平与临床和病理分期显著相关，随后被证明与NSCLC的发生和进展有关。总之，TβRII和DPC4/Smad4可能通过TGF-β信号通路在NSCLC的肿瘤发生、分化和进展中发挥重要作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd44/4247285/59b29022efe1/ETM-09-01-0227-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd44/4247285/fb35b5e3748c/ETM-09-01-0227-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd44/4247285/0e6e671ce44c/ETM-09-01-0227-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd44/4247285/59b29022efe1/ETM-09-01-0227-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd44/4247285/fb35b5e3748c/ETM-09-01-0227-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd44/4247285/0e6e671ce44c/ETM-09-01-0227-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd44/4247285/59b29022efe1/ETM-09-01-0227-g02.jpg

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