枯草芽孢杆菌的细胞色素氧化酶：影响细胞色素aa3的基因定位及其在突变株中被细胞色素o取代的研究

The cytochrome oxidases of Bacillus subtilis: mapping of a gene affecting cytochrome aa3 and its replacement by cytochrome o in a mutant strain.

作者信息

James W S, Gibson F, Taroni P, Poole R K

机构信息

Biochemistry Department, University of Oxford, U.K.

出版信息

FEMS Microbiol Lett. 1989 Apr;49(2-3):277-81. doi: 10.1016/0378-1097(89)90053-0.

DOI:10.1016/0378-1097(89)90053-0

PMID:2545519

Abstract

A mutant of Bacillus subtilis has been isolated that fails to grow on succinate as the source of carbon, yet grows on glucose. Intact cells of the mutant and cytoplasmic membranes derived therefrom lack cytochromes a and a3 but contain a cytochrome o-like pigment, which forms a photodissociable compound with CO and is reactive with oxygen. The mutation in the genome has been located and lies at about 130 degrees on the chromosomal map between the metC and pyrD loci. The designation cox is suggested for this gene.

摘要

已分离出一种枯草芽孢杆菌突变体，该突变体不能以琥珀酸盐作为碳源生长，但能在葡萄糖上生长。该突变体的完整细胞及其衍生的细胞质膜缺乏细胞色素a和a3，但含有一种类似细胞色素o的色素，该色素能与一氧化碳形成光解离化合物并与氧气发生反应。基因组中的突变已定位，位于染色体图谱上metC和pyrD基因座之间约130度处。建议将该基因命名为cox。