Shen Li-Juan, Guan Yun-Yan, Wu Xi-Ping, Wang Qian, Wang Liang, Xiao Tao, Wu Hai-Rong, Wang Jin-Gui
Department of ICU, Wuxi Hospital of traditional Chinese Medicine, Nanjing University of Chinese Medicine Affiliated Wuxi Hospital, No. 8, South West Road, Wuxi 214071, Jiangsu, China.
Department of ICU, Wuxi Hospital of traditional Chinese Medicine, Nanjing University of Chinese Medicine Affiliated Wuxi Hospital, No. 8, South West Road, Wuxi 214071, Jiangsu, China.
Clin Res Hepatol Gastroenterol. 2015 Apr;39(2):230-6. doi: 10.1016/j.clinre.2014.10.002. Epub 2014 Nov 11.
To investigate the use of citrulline as an indicator for diagnosing septic acute intestinal dysfunction (SAID) in a rat model.
SD rats were divided into three groups: a normal group (A), a model group (B), and a glutamine group (C). Group B was divided into a 36-h group (B1) and a 72-h feeding group (B2). The concentrations of serum citrulline, intestinal fatty acid-binding protein (I-FABP) and intestinal glutamine and histopathological changes were measured.
The lengths of the villus and thicknesses of the mucosal layer in groups B1, B2 and C were significantly different from those in group A. Citrulline concentrations in groups B1, B2 and C were lower than in group A; the serum concentrations in group C were significantly greater than in groups B1 and B2. The I-FABP levels of groups B1, B2 and C were higher than group A; I-FABP levels of groups B1 and B2 were higher than group C. Intestinal glutamine levels of groups B1 and B2 were lower than groups A and C. The serum citrulline of group C was negatively correlated with I-FABP and Chiu's score.
Serum citrulline could be used as the diagnostic indicator of SAID.
在大鼠模型中研究瓜氨酸作为诊断脓毒症急性肠功能障碍（SAID）指标的应用。
将SD大鼠分为三组：正常组（A组）、模型组（B组）和谷氨酰胺组（C组）。B组再分为36小时组（B1组）和72小时喂养组（B2组）。检测血清瓜氨酸、肠脂肪酸结合蛋白（I-FABP）、肠谷氨酰胺浓度及组织病理学变化。
B1组、B2组和C组的绒毛长度和黏膜层厚度与A组有显著差异。B1组、B2组和C组的瓜氨酸浓度低于A组；C组血清浓度显著高于B1组和B2组。B1组、B2组和C组的I-FABP水平高于A组；B1组和B2组的I-FABP水平高于C组。B1组和B2组的肠谷氨酰胺水平低于A组和C组。C组血清瓜氨酸与I-FABP及Chiu评分呈负相关。
血清瓜氨酸可作为SAID的诊断指标。
Zotero 插件