Lin Chengyuan, Jiang Xue, Hu Guangfu, Ko Wendy K W, Wong Anderson O L
School of Biological Sciences, University of Hong Kong, Hong Kong; YMU-HKBU Joint Laboratory of Traditional Natural Medicine, Yunnan Minzu University, Kunming, China.
School of Biological Sciences, University of Hong Kong, Hong Kong.
Mol Cell Endocrinol. 2015 Jan 5;399:267-83. doi: 10.1016/j.mce.2014.10.010. Epub 2014 Oct 16.
Prolactin (PRL), a pituitary hormone with diverse functions, is well-documented to be under the control of both hypothalamic and peripheral signals. Intrapituitary modulation of PRL expression via autocrine/paracrine mechanisms has also been reported, but similar information is still lacking in lower vertebrates. To shed light on autocrine/paracrine regulation of PRL in fish model, grass carp PRL was cloned and its expression in the carp pituitary has been confirmed. In grass carp pituitary cells, local secretion of PRL could suppress PRL release with concurrent rises in PRL production and mRNA levels. Paracrine stimulation by growth hormone (GH) was found to up- regulate PRL secretion, PRL production and PRL transcript expression, whereas the opposite was true for the local actions of luteinizing hormone (LH). Apparently, local interactions of PRL, GH and LH via autocrine/paracrine mechanisms could modify PRL production in carp pituitary cells through differential regulation of PRL mRNA stability and gene transcription.
催乳素（PRL）是一种具有多种功能的垂体激素，有充分证据表明其受下丘脑和外周信号的控制。垂体内部通过自分泌/旁分泌机制对PRL表达进行调节的情况也有报道，但在低等脊椎动物中仍缺乏类似信息。为了阐明鱼类模型中PRL的自分泌/旁分泌调节，克隆了草鱼PRL并证实了其在鲤鱼垂体中的表达。在草鱼垂体细胞中，PRL的局部分泌可抑制PRL释放，同时PRL产量和mRNA水平升高。发现生长激素（GH）的旁分泌刺激可上调PRL分泌、PRL产量和PRL转录本表达，而促黄体生成素（LH）的局部作用则相反。显然，PRL、GH和LH通过自分泌/旁分泌机制的局部相互作用可通过对PRL mRNA稳定性和基因转录的差异调节来改变鲤鱼垂体细胞中PRL的产生。
