DBT-IOC Centre for Advanced Bio-Energy Research, Indian Oil Corporation Ltd, R&D Centre, Sector-13, Faridabad 121007, India.
DBT-IOC Centre for Advanced Bio-Energy Research, Indian Oil Corporation Ltd, R&D Centre, Sector-13, Faridabad 121007, India.
Bioresour Technol. 2015 Jan;175:291-7. doi: 10.1016/j.biortech.2014.10.110. Epub 2014 Oct 28.
Hydrogen producing bacteria IODB-O3 was isolated from sludge and identified as Clostridium sp. by 16S rDNA gene analysis. In this study, biohydrogen production process was developed using low-cost agro-waste. Maximum H2 was produced at 37°C and pH 8.5. Maximum H2 yield was obtained 2.54±0.2mol-H2/mol-reducing sugar from wheat straw pre-hydrolysate (WSPH) and 2.61±0.1mol-H2/mol-reducing sugar from pre-treated wheat straw enzymatic-hydrolysate (WSEH). The cumulative H2 production (ml/L), 3680±105 and 3270±100, H2 production rate (ml/L/h), 153±5 and 136±5, and specific H2 production (ml/g/h), 511±5 and 681±10 with WSPH and WSEH were obtained, respectively. Biomass pre-treatment via steam-explosion generates ample amount of WSPH which remains unutilized for bioethanol production due to non-availability of efficient C5-fermenting microorganisms. This study shows that Clostridium sp. IODB-O3 is capable of utilizing WSPH efficiently for biohydrogen production. This would lead to reduced economic constrain on the overall cellulosic ethanol process and also establish a sustainable biohydrogen production process.
从污泥中分离出产氢细菌 IODB-O3，并通过 16S rDNA 基因分析鉴定为梭菌属。在这项研究中，利用低成本的农业废弃物开发了生物制氢工艺。在 37°C 和 pH 8.5 的条件下，最大产氢量达到 2.54±0.2mol-H2/mol-还原糖，来自小麦秸秆预水解物（WSPH）和预处理小麦秸秆酶解物（WSEH）的最大产氢量分别为 2.61±0.1mol-H2/mol-还原糖。用 WSPH 和 WSEH 获得的累积产氢量（ml/L）分别为 3680±105 和 3270±100，产氢率（ml/L/h）分别为 153±5 和 136±5，比产氢速率（ml/g/h）分别为 511±5 和 681±10。由于缺乏有效的 C5 发酵微生物，蒸汽爆破产生的大量 WSPH 尚未用于生物乙醇生产，仍未得到利用。本研究表明，Clostridium sp. IODB-O3 能够有效地利用 WSPH 进行生物制氢。这将降低整体纤维素乙醇工艺的经济制约，并建立可持续的生物制氢工艺。
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