在鸡背根神经节的神经元前体细胞中，质子诱导的钠离子电流在电压依赖性钠离子和钙离子电流之前产生。

Proton-induced Na+ current develops prior to voltage-dependent Na+ and Ca2+ currents in neuronal precursor cells from chick dorsal root ganglion.

作者信息

Gottmann K, Dietzel I D, Lux H D, Ruedel C

机构信息

Max-Planck-Institute for Psychiatry, Department of Neurophysiology, Planegg-Martinsried F.R.G.

出版信息

Neurosci Lett. 1989 Apr 24;99(1-2):90-4. doi: 10.1016/0304-3940(89)90270-x.

DOI:10.1016/0304-3940(89)90270-x

PMID:2546110

Abstract

Proton-induced Na+ currents (INa(H] were measured in precursor cells from chick dorsal root ganglia with whole-cell patch-clamp recording. Precursor cells were isolated using the method of Rohrer et al. (EMBO. J., 4 (1985) 1709-1714) and fast pH changes were applied with a technique developed by Davies et al. (J. Physiol. (Lond.), 400 (1988) 159-187). Proton-induced transient Na+ currents showing the same properties as in more mature neurons could be elicited already early in differentiation, before high-voltage activated Ca2+ currents and voltage-dependent Na+ currents develop.

摘要

采用全细胞膜片钳记录技术，在鸡背根神经节的前体细胞中测量质子诱导的钠离子电流（INa(H)）。前体细胞采用Rohrer等人（《欧洲分子生物学组织杂志》，4 (1985) 1709 - 1714）的方法分离，快速pH变化采用Davies等人（《生理学杂志》(伦敦)，400 (1988) 159 - 187）开发的技术施加。在高电压激活的钙离子电流和电压依赖性钠离子电流出现之前，分化早期就能引发具有与更成熟神经元相同特性的质子诱导瞬时钠离子电流。