胎儿期母体甲状腺素水平过低对成年子代大鼠大脑中钙调蛋白调节的磷酸酶活性的影响。

Effect of maternal hypothyroxinaemia during fetal life on the calmodulin-regulated phosphatase activity in the brain of the adult progeny in the rat.

作者信息

Ruiz de Elvira M C, Sinha A K, Pickard M, Ballabio M, Hubank M, Ekins R P

机构信息

Department of Molecular Endocrinology, University College and Middlesex School of Medicine, London.

出版信息

J Endocrinol. 1989 May;121(2):331-5. doi: 10.1677/joe.0.1210331.

DOI:10.1677/joe.0.1210331

PMID:2547007

Abstract

Calmodulin-regulated phosphatase activity was measured in the brain of 2-month-old rats born from hypothyroid and normal dams, using a fluorometric enzyme assay developed for this purpose. Calmodulin content was measured in the same brain regions by radioimmunoassay. Significant differences between groups in weight and protein content, basal phosphatase and calmodulin-regulated phosphatase activity were found. The brain region most affected was the cerebellum, where basal and calmodulin-regulated phosphatase activities, and protein content were increased. The data point towards a lasting effect of maternal hypothyroxinaemia on the brain function of the progeny.

摘要

使用为此目的开发的荧光酶测定法，测定了甲状腺功能减退和正常母鼠所生2月龄大鼠大脑中的钙调蛋白调节磷酸酶活性。通过放射免疫测定法在相同脑区测量钙调蛋白含量。发现各组之间在体重、蛋白质含量、基础磷酸酶和钙调蛋白调节磷酸酶活性方面存在显著差异。受影响最严重的脑区是小脑，其中基础和钙调蛋白调节的磷酸酶活性以及蛋白质含量均增加。数据表明母体甲状腺素血症不足对后代脑功能有持久影响。