Povilenis P I, Lubis A A, Vaĭshvila R I, Kulakauskas S T, Ianulaĭtis A A
Genetika. 1989 Apr;25(4):753-5.
Experiments on transformation of Escherichia coli K-12 cells by plasmids carrying RM systems with different recognition sites containing 5-methylcytosine have shown that the gene mcrB determines the function of restriction. The data obtained made it possible to believe that E. coli possesses no restriction system recognizing specifically cytosine methylated in position 4.
用携带具有不同识别位点(含5 - 甲基胞嘧啶)的RM系统的质粒对大肠杆菌K - 12细胞进行转化实验表明,mcrB基因决定限制功能。所获得的数据使人有可能相信,大肠杆菌不存在特异性识别在第4位甲基化胞嘧啶的限制系统。