楔形蛤蜊（Donax cuneatus，林奈）蛋白质水解物的自由基清除及氨基酸分析
Radical scavenging and amino acid profiling of wedge clam, Donax cuneatus (Linnaeus) protein hydrolysates.
作者信息
Nazeer R A, Saranya M A V, Naqash Shabeena Yousuf
机构信息
Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, 603 203 Chennai, Tamilnadu India.
出版信息
J Food Sci Technol. 2014 Dec;51(12):3942-8. doi: 10.1007/s13197-012-0894-6. Epub 2012 Nov 16.
Body, foot and viscera of Donax cuneatus (Linnaeus) were hydrolyzed using commercial proteases (pepsin, trypsin and papain) and tested for their antioxidant activity by DPPH scavenging ability and reducing power assays. In comparison between all the hydrolysates, papain viscera (28.513 ± 0.165 & 0.186 ± 0.008) and foot (33.567 ± 0.132 & 0.166 ± 0.013) hydrolysates showed highest DPPH and reducing power ability respectively. The active hydrolysates were purified with DEAE- cellulose followed by Sephadex G-25 columns connected to FPLC. Further, the isolated active fractions were loaded onto HPLC for their amino acid profiling and found with the presence of potential amino acids viz., histidine, cysteine, alanine etc. These results suggest that the isolated antioxidant peptide from viscera and foot hydrolysate of D. cuneatus can be used in treating human diseases where free radicals and oxidative damage are involved.
使用商业蛋白酶（胃蛋白酶、胰蛋白酶和木瓜蛋白酶）对楔形多毛蟹（林奈）的身体、足部和内脏进行水解，并通过DPPH清除能力和还原能力测定来测试其抗氧化活性。在所有水解产物之间的比较中，木瓜蛋白酶水解的内脏（28.513±0.165和0.186±0.008）和足部（33.567±0.132和0.166±0.013）水解产物分别表现出最高的DPPH清除能力和还原能力。活性水解产物先用DEAE-纤维素纯化，然后通过连接到快速蛋白质液相色谱的葡聚糖G-25柱进行纯化。此外，将分离出的活性级分加载到高效液相色谱上进行氨基酸分析，发现存在潜在的氨基酸，即组氨酸、半胱氨酸、丙氨酸等。这些结果表明，从楔形多毛蟹的内脏和足部水解产物中分离出的抗氧化肽可用于治疗涉及自由基和氧化损伤问题的人类疾病。
Zotero 插件