Choi Yoon Seok, Bae Chang Hoon, Song Si-Youn, Kim Yong-Dae
Rhinology. 2014 Dec;52(4):406-12. doi: 10.4193/Rhino13.189.
The variety of beneficial effects of green tea has been reported. Epigallocatechin-3-gallate (EGCG) is a major component of green tea extract. The biological activity of EGCG includes anti-allergic and anti-inflammatory activities. However, the precise effect of EGCG on the allergic airway inflammation has not been fully defined.
In lung tissues of an asthma model-mouse, and nasal epithelial cells of patients with allergic rhinitis, the effect and brief signaling pathway of EGCG on MUC5B expression were investigated using real-time polymerase chain reaction, immunohistochemical, and immunoblot analysis.
In the asthma model-mouse, mucus production, MUC5B expression, p38 mitogen-activated protein kinase (MAPK) and matrix metalloprotease (MMP)-9 expression of the asthma group were significantly higher than in the control group. Extracellular signal related kinase (ERK)1/2 MAPK expression did not change. Mucus production, MUC5B expression, p38 MAPK expression, and MMP-9 expression of the asthma + EGCG group were significantly lower than in the asthma group. In the nasal epithelial cells of patients with allergic rhinitis, EGCG significantly decreased phorbol 12-myristate 13-acetate (PMA)-induced MUC5B and MMP-9 expression.
These results suggest that EGCG reduces mucin expression in the asthma model-mouse and nasal epithelial cells of patients with allergic inflammation.
绿茶的多种有益功效已有报道。表没食子儿茶素-3-没食子酸酯（EGCG）是绿茶提取物的主要成分。EGCG的生物活性包括抗过敏和抗炎活性。然而，EGCG对过敏性气道炎症的确切作用尚未完全明确。
在哮喘模型小鼠的肺组织以及过敏性鼻炎患者的鼻上皮细胞中，采用实时聚合酶链反应、免疫组织化学和免疫印迹分析研究EGCG对MUC5B表达的作用及简要信号通路。
在哮喘模型小鼠中，哮喘组的黏液分泌、MUC5B表达、p38丝裂原活化蛋白激酶（MAPK）和基质金属蛋白酶（MMP）-9表达显著高于对照组。细胞外信号调节激酶（ERK）1/2 MAPK表达未发生变化。哮喘+EGCG组的黏液分泌、MUC5B表达、p38 MAPK表达和MMP-9表达显著低于哮喘组。在过敏性鼻炎患者的鼻上皮细胞中，EGCG显著降低佛波酯12-肉豆蔻酸酯13-乙酸酯（PMA）诱导的MUC5B和MMP-9表达。
这些结果表明，EGCG可降低哮喘模型小鼠和过敏性炎症患者鼻上皮细胞中的黏蛋白表达。
