Hsia Kan-Tai, Liu Chung-Ji, Mar Kwei, Lin Li-Han, Lin Chun-Shu, Cheng Ming-Fang, Lee Herng-Sheng, Chiu Shang-Yi
Institute of Oral Biology, School of Dentistry, National Yang-Ming University, Taipei, Taiwan.
Department of Oral and Maxillofacial Surgery, Taipei Mackay Memorial Hospital, Taipei, Taiwan.
Head Neck. 2016 Apr;38(4):550-9. doi: 10.1002/hed.23927. Epub 2015 Jun 16.
Apurinic/apyrimidinic endonuclease 1/redox factor-1 (APE1/Ref-1) is a multifunctional protein involved in DNA repair and redox signaling. The purpose of this study was to investigate the relationship between APE1/Ref-1 expression and clinicopathological features, survival, and treatment response in patients with oral squamous cell carcinoma (OSCC) and cell lines.
APE1/Ref-1 expression in OSCC was evaluated by immunohistochemistry, and its relationship to patient outcomes and treatment response was assessed statistically. The effects of stable short hairpin (sh)RNA-mediated knockdown of APE1/Ref-1 on cell survival, migration, and chemoradiation sensitivity were determined in OSCC cell lines.
APE1/Ref-1 immunostaining was correlated with positive lymph node status, and higher APE1/Ref-1 expression was significantly associated with poor prognosis and reduced treatment response. Consistent with the clinical studies, APE1/Ref-1 expression in OSCC cell lines was implicated in the regulation of migration and cisplatin-induced apoptosis.
Elevated APE1/Ref-1 expression may be used to predict poor survival and may confer chemoresistance in OSCC.
脱嘌呤/脱嘧啶内切酶1/氧化还原因子-1（APE1/Ref-1）是一种参与DNA修复和氧化还原信号传导的多功能蛋白。本研究旨在探讨APE1/Ref-1表达与口腔鳞状细胞癌（OSCC）患者及细胞系的临床病理特征、生存率和治疗反应之间的关系。
采用免疫组织化学法评估OSCC中APE1/Ref-1的表达，并对其与患者预后和治疗反应的关系进行统计学评估。在OSCC细胞系中确定稳定短发夹（sh）RNA介导的APE1/Ref-1敲低对细胞存活、迁移和放化疗敏感性的影响。
APE1/Ref-1免疫染色与阳性淋巴结状态相关，APE1/Ref-1表达升高与预后不良和治疗反应降低显著相关。与临床研究一致，OSCC细胞系中APE1/Ref-1的表达与迁移调节和顺铂诱导的细胞凋亡有关。
APE1/Ref-1表达升高可用于预测OSCC患者的不良生存，并可能赋予其化疗抗性。
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