一种生长素诱导型DNA结合蛋白基因的功能分析
Functional analysis of an auxin-inducible DNA-binding protein gene.
作者信息
Bernstein Any, Mangeon Amanda, Almeida-Engler Janice, Engler Gilbert, Van Montagu Marc, Sachetto-Martins Gilberto, de Oliveira Dulce Eleonora
机构信息
a Laboratório de Genômica Funcional e Transdução de Sinal; Departamento de Genética ; Universidade Federal do Rio de Janeiro ; Brazil.
出版信息
Plant Signal Behav. 2015;10(1):e977706. doi: 10.4161/15592324.2014.977706.
Over the past decades, several studies indicate a correlation between the phytohormone auxin and cell division. The molecular players of this signaling pathway are now being uncovered. DNA Binding Protein1 from Arabidopsis (AtDBP1) is an auxin-inducible gene able to bind DNA non-specifically. In this work the tissue-expression pattern of this gene was investigated. Promoter-GUS analysis demonstrated that the AtDBP1 promoter is active in regions exhibiting intense cell division such as meristems and nematode feeding sites. Also, the promoter expression was modulated upon incubation with cell cycle blockers, indicating a potential role in cell division for this gene. Lastly, AtDBP1 antisense plants presented a higher insensitivity to auxin, and interfered negatively with auxin-induced callus formation and reduced apical dominance.
在过去几十年中，多项研究表明植物激素生长素与细胞分裂之间存在关联。目前，这一信号通路的分子作用因子正在被揭示。拟南芥DNA结合蛋白1（AtDBP1）是一个能非特异性结合DNA的生长素诱导型基因。在本研究中，对该基因的组织表达模式进行了研究。启动子-GUS分析表明，AtDBP1启动子在分生组织和线虫取食位点等细胞分裂活跃的区域具有活性。此外，用细胞周期阻滞剂处理后，启动子表达受到调控，表明该基因在细胞分裂中可能发挥作用。最后，AtDBP1反义植物对生长素的敏感性更高，对生长素诱导的愈伤组织形成产生负干扰，并降低了顶端优势。
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