血小板衍生生长因子刺激C3H成纤维细胞中异质性多磷酸肌醇代谢及磷酸盐摄取。
Platelet-derived-growth-factor-stimulated heterogeneous polyphosphoinositide metabolism and phosphate uptake in C3H fibroblasts.
作者信息
Holmsen H, Male R, Rongved S, Langeland N, Lillehaug J
机构信息
Department of Biochemistry, University of Bergen, Norway.
出版信息
Biochem J. 1989 Jun 1;260(2):589-92. doi: 10.1042/bj2600589.
Abstract
Pig platelet-derived growth factor (PDGF) increased the rate of [32P]Pi uptake by murine fibroblasts, resulting in a 3-9-fold elevation of the specific radioactivity of ATP, PtdInsP, PtdInsP2, PtdIns and phosphatidic acid. The specific radioactivity was 10-60-fold higher in ATP than in the four phospholipids. These substances are therefore not in metabolic equilibrium, which complicates determination of inositol phospholipid turnover.
摘要
猪血小板衍生生长因子（PDGF）提高了小鼠成纤维细胞对[32P]Pi的摄取率，导致ATP、磷脂酰肌醇磷酸（PtdInsP）、磷脂酰肌醇二磷酸（PtdInsP2）、磷脂酰肌醇（PtdIns）和磷脂酸的比放射性提高了3至9倍。ATP的比放射性比四种磷脂高10至60倍。因此，这些物质并非处于代谢平衡状态，这使得肌醇磷脂周转率的测定变得复杂。
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本文引用的文献
1
Early changes in phosphatidylinositol and arachidonic acid metabolism in quiescent swiss 3T3 cells stimulated to divide by platelet-derived growth factor.
J Biol Chem. 1981 Dec 10;256(23):12329-35.
2
Determination of levels of glycolytic intermediates and nucleotides in platelets by pulse-labeling with [32P]orthophosphate.
Anal Biochem. 1983 May;131(1):266-72. doi: 10.1016/0003-2697(83)90165-3.
3
Reduction of epidermal growth factor receptor affinity by heterologous ligands: evidence for a mechanism involving the breakdown of phosphoinositides and the activation of protein kinase C.
Biochem Biophys Res Commun. 1984 Aug 30;123(1):377-84. doi: 10.1016/0006-291x(84)90424-8.
4
Inositol trisphosphate formation and calcium mobilization in Swiss 3T3 cells in response to platelet-derived growth factor.
Biochem J. 1984 Aug 15;222(1):195-201. doi: 10.1042/bj2220195.
5
Transport changes rapidly initiated by serum addition to "contact inhibited" 3T3 cells.
Proc Natl Acad Sci U S A. 1969 Nov;64(3):1049-56. doi: 10.1073/pnas.64.3.1049.
6
Synergism between thrombin and epinephrine in human platelets: different dose-response relationships for aggregation and dense granule secretion.
Thromb Haemost. 1985 Oct 30;54(3):680-3.
7
Early changes in inositol lipids and their metabolites induced by platelet-derived growth factor in quiescent Swiss mouse 3T3 cells.
Biochem J. 1985 Nov 15;232(1):99-109. doi: 10.1042/bj2320099.
8
Effects of PDGF on inositol phosphates, Ca2+, and contraction of mesangial cells.
Am J Physiol. 1987 Sep;253(3 Pt 2):F458-63. doi: 10.1152/ajprenal.1987.253.3.F458.
9
Effects of confluence on phosphate transport capacity in cultured renal cell lines.
J Cell Physiol. 1988 Apr;135(1):122-6. doi: 10.1002/jcp.1041350117.
10
Pathway of phospholipase C activation initiated with platelet-derived growth factor is different from that initiated with vasopressin and bombesin.
J Biol Chem. 1988 Sep 15;263(26):12970-6.
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