血小板衍生生长因子刺激C3H成纤维细胞中异质性多磷酸肌醇代谢及磷酸盐摄取。
Platelet-derived-growth-factor-stimulated heterogeneous polyphosphoinositide metabolism and phosphate uptake in C3H fibroblasts.
作者信息
Holmsen H, Male R, Rongved S, Langeland N, Lillehaug J
机构信息
Department of Biochemistry, University of Bergen, Norway.
出版信息
Biochem J. 1989 Jun 1;260(2):589-92. doi: 10.1042/bj2600589.
Abstract
Pig platelet-derived growth factor (PDGF) increased the rate of [32P]Pi uptake by murine fibroblasts, resulting in a 3-9-fold elevation of the specific radioactivity of ATP, PtdInsP, PtdInsP2, PtdIns and phosphatidic acid. The specific radioactivity was 10-60-fold higher in ATP than in the four phospholipids. These substances are therefore not in metabolic equilibrium, which complicates determination of inositol phospholipid turnover.
摘要
猪血小板衍生生长因子（PDGF）提高了小鼠成纤维细胞对[32P]Pi的摄取率，导致ATP、磷脂酰肌醇磷酸（PtdInsP）、磷脂酰肌醇二磷酸（PtdInsP2）、磷脂酰肌醇（PtdIns）和磷脂酸的比放射性提高了3至9倍。ATP的比放射性比四种磷脂高10至60倍。因此，这些物质并非处于代谢平衡状态，这使得肌醇磷脂周转率的测定变得复杂。
相似文献
1
Platelet-derived-growth-factor-stimulated heterogeneous polyphosphoinositide metabolism and phosphate uptake in C3H fibroblasts.血小板衍生生长因子刺激C3H成纤维细胞中异质性多磷酸肌醇代谢及磷酸盐摄取。
Biochem J. 1989 Jun 1;260(2):589-92. doi: 10.1042/bj2600589.
2
Early changes in inositol lipids and their metabolites induced by platelet-derived growth factor in quiescent Swiss mouse 3T3 cells.血小板衍生生长因子诱导静止的瑞士小鼠3T3细胞中肌醇脂质及其代谢产物的早期变化。
Biochem J. 1985 Nov 15;232(1):99-109. doi: 10.1042/bj2320099.
3
Platelet-derived growth factor stimulates rapid polyphosphoinositide breakdown in fetal human fibroblasts.血小板衍生生长因子刺激人胎儿成纤维细胞中多磷酸肌醇快速分解。
J Cell Physiol. 1985 Sep;124(3):391-6. doi: 10.1002/jcp.1041240306.
4
Inositol phospholipid metabolism in human platelets stimulated by ADP.ADP刺激下的人血小板中的肌醇磷脂代谢
Eur J Biochem. 1990 Oct 24;193(2):521-8. doi: 10.1111/j.1432-1033.1990.tb19367.x.
5
Comparison of PDGF-AA- and PDGF-BB-induced phosphoinositide formation in human and mouse fibroblasts.血小板衍生生长因子-AA（PDGF-AA）和血小板衍生生长因子-BB（PDGF-BB）诱导人及小鼠成纤维细胞中磷酸肌醇形成的比较。
Exp Cell Res. 1994 Apr;211(2):286-95. doi: 10.1006/excr.1994.1089.
6
Evidence that the inositol phospholipids are necessary for exocytosis. Loss of inositol phospholipids and inhibition of secretion in permeabilized cells caused by a bacterial phospholipase C and removal of ATP.肌醇磷脂对于胞吐作用必不可少的证据。细菌磷脂酶C导致的肌醇磷脂丧失以及通透细胞中ATP去除所引起的分泌抑制。
Biochem J. 1990 May 15;268(1):15-25. doi: 10.1042/bj2680015.
7
Neomycin inhibits PDGF-induced IP3 formation and DNA synthesis but not PDGF-stimulated uptake of inorganic phosphate in C3H/10T1/2 fibroblasts.
Biochim Biophys Acta. 1990 Sep 1;1054(2):207-12. doi: 10.1016/0167-4889(90)90242-6.
8
Phosphate turnover of phosphatidylinositol in resting and thrombin-stimulated platelets.静息和凝血酶刺激的血小板中磷脂酰肌醇的磷酸周转
Biochim Biophys Acta. 1986 Nov 28;889(2):183-91. doi: 10.1016/0167-4889(86)90103-5.
9
Regulation of phosphoinositide phosphorylation in Swiss 3T3 cells stimulated by platelet-derived growth factor.
J Biol Chem. 1987 Jan 25;262(3):1105-10.
10
Rates of production and consumption of phosphatidic acid upon thrombin stimulation of human platelets.凝血酶刺激人血小板后磷脂酸的生成和消耗速率。
Eur J Biochem. 1988 May 16;174(1):75-9. doi: 10.1111/j.1432-1033.1988.tb14064.x.
引用本文的文献
1
Choline derived from the phosphatidylcholine specific phospholipase D is not directly available for the CDP choline pathway in phorbol ester-treated C3H10T1/2 Cl 8 fibroblasts.在佛波酯处理的C3H10T1/2 Cl 8成纤维细胞中，源自磷脂酰胆碱特异性磷脂酶D的胆碱不能直接用于CDP胆碱途径。
Mol Cell Biochem. 1998 Oct;187(1-2):147-54. doi: 10.1023/a:1006813524791.
本文引用的文献
1
2
Determination of levels of glycolytic intermediates and nucleotides in platelets by pulse-labeling with [32P]orthophosphate.通过用[32P]正磷酸盐脉冲标记法测定血小板中糖酵解中间产物和核苷酸的水平。
Anal Biochem. 1983 May;131(1):266-72. doi: 10.1016/0003-2697(83)90165-3.
3
Reduction of epidermal growth factor receptor affinity by heterologous ligands: evidence for a mechanism involving the breakdown of phosphoinositides and the activation of protein kinase C.异源配体降低表皮生长因子受体亲和力：涉及磷酸肌醇分解和蛋白激酶C激活机制的证据
Biochem Biophys Res Commun. 1984 Aug 30;123(1):377-84. doi: 10.1016/0006-291x(84)90424-8.
4
Inositol trisphosphate formation and calcium mobilization in Swiss 3T3 cells in response to platelet-derived growth factor.瑞士3T3细胞中肌醇三磷酸的形成及钙动员对血小板衍生生长因子的响应
Biochem J. 1984 Aug 15;222(1):195-201. doi: 10.1042/bj2220195.
5
Transport changes rapidly initiated by serum addition to "contact inhibited" 3T3 cells.向“接触抑制”的3T3细胞中添加血清后迅速引发转运变化。
Proc Natl Acad Sci U S A. 1969 Nov;64(3):1049-56. doi: 10.1073/pnas.64.3.1049.
6
7
Early changes in inositol lipids and their metabolites induced by platelet-derived growth factor in quiescent Swiss mouse 3T3 cells.血小板衍生生长因子诱导静止的瑞士小鼠3T3细胞中肌醇脂质及其代谢产物的早期变化。
Biochem J. 1985 Nov 15;232(1):99-109. doi: 10.1042/bj2320099.
8
Effects of PDGF on inositol phosphates, Ca2+, and contraction of mesangial cells.
Am J Physiol. 1987 Sep;253(3 Pt 2):F458-63. doi: 10.1152/ajprenal.1987.253.3.F458.
9
Effects of confluence on phosphate transport capacity in cultured renal cell lines.汇合对培养的肾细胞系中磷酸盐转运能力的影响。
J Cell Physiol. 1988 Apr;135(1):122-6. doi: 10.1002/jcp.1041350117.
Zotero 插件