大鼠腹膜肥大细胞对白三烯D4的代谢

Metabolism of leukotriene D4 by rat peritoneal mast cells.

作者信息

Koyano H, Yamashita T

机构信息

Dept. of Biochemistry, School of Medicine, Juntendo University, Tokyo, Japan.

出版信息

Prostaglandins. 1989 Aug;38(2):215-26. doi: 10.1016/0090-6980(89)90084-1.

DOI:10.1016/0090-6980(89)90084-1

PMID:2549577

Abstract

Metabolism of sulfidopeptide leukotrienes, leukotrienes (LT) C4 and D4 by rat peritoneal mast cells was studied. Rat peritoneal mast cells converted LTD4 to LTE4 but not LTC4 to LTD4. The LTD4-metabolizing activity was equally distributed on the cell surface and inside cells, but not released to the extracellular milieu even when a considerable portion of histamine and the secretory granule enzymes were released. Among various enzyme inhibitors examined, o-phenanthroline, a metal chelator, and dithiothreitol significantly suppressed the LTD4-metabolizing activity of mast cell. These results would suggest that some metalloenzyme located on the cell surface is involved in the conversion of LTD4 to LTE4 by rat peritoneal mast cells.

摘要

研究了大鼠腹膜肥大细胞对硫肽白三烯、白三烯（LT）C4和D4的代谢。大鼠腹膜肥大细胞可将LTD4转化为LTE4，但不能将LTC4转化为LTD4。LTD4代谢活性在细胞表面和细胞内均匀分布，即使相当一部分组胺和分泌颗粒酶被释放，也不会释放到细胞外环境中。在检测的各种酶抑制剂中，金属螯合剂邻菲罗啉和二硫苏糖醇显著抑制肥大细胞的LTD4代谢活性。这些结果表明，位于细胞表面的某些金属酶参与了大鼠腹膜肥大细胞将LTD4转化为LTE4的过程。