Simoni M, Khan S A, De Geyter C, Nieschlag E
Max Planck Clinical Research Unit for Reproductive Medicine, Münster, FRG.
Acta Endocrinol (Copenh). 1989 Aug;121(2):265-9. doi: 10.1530/acta.0.1210265.
Effects of serum from pregnant women on basal and FSH (or cAMP) stimulated aromatase activity of immature rat Sertoli cells in primary culture were studied. Pregnancy serum caused a dose-dependent stimulation of Sertoli cell aromatase activity and the response curves were parallel to those obtained with human FSH. This stimulatory (FSH-like) activity increased progressively during pregnancy, with a sharp drop immediately after delivery. However, the FSH-like bioactivity was not associated with immunoreactive FSH when a specific radioimmunoassay was employed. On the other hand, serum from pregnant women caused a dose-dependent inhibition of FSH and dibutyryl-cAMP-stimulated aromatase activity. These data suggest that human pregnancy serum contains factor(s) which may stimulate basal aromatase activity of Sertoli cells and may inhibit FSH-induced aromatase activity. These factors, most probably of placental origin, may play a role in the regulation of estrogen production during gestation.
研究了孕妇血清对原代培养的未成熟大鼠支持细胞基础及促卵泡激素（FSH）（或环磷酸腺苷，cAMP）刺激的芳香化酶活性的影响。妊娠血清对支持细胞芳香化酶活性有剂量依赖性刺激作用，且反应曲线与用人FSH获得的曲线平行。这种刺激（类FSH）活性在妊娠期间逐渐增加，分娩后立即急剧下降。然而，当采用特异性放射免疫测定时，类FSH生物活性与免疫反应性FSH无关。另一方面，孕妇血清对FSH和二丁酰cAMP刺激的芳香化酶活性有剂量依赖性抑制作用。这些数据表明，人妊娠血清含有可能刺激支持细胞基础芳香化酶活性并可能抑制FSH诱导的芳香化酶活性的因子。这些因子很可能来源于胎盘，可能在妊娠期雌激素产生的调节中起作用。
