Hiraishi Noriko, Tochio Naoya, Kigawa Takanori, Otsuki Masayuki, Tagami Junji
Cariology and Operative Dentistry, Department of Oral Health Sciences, Graduate School, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan.
Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, 1-3-2 Kagamiyama, Higashi-Hiroshima 739-8511, Japan.
Dent Mater. 2015 Feb;31(2):88-92. doi: 10.1016/j.dental.2014.11.005. Epub 2014 Dec 11.
2-Hydroxyethylmethacrylate (HEMA) diffuses in wet dentin and promotes adhesion during dentin priming and bonding. We have investigated the molecular level interaction between HEMA and a collagen model by using saturation transfer difference (STD) NMR.
The binding of HEMA to collagen was preliminarily investigated by suspending demineralized human dentin powders in a 4mM HEMA solution for 1h and measuring the decrease in the HEMA concentration on a spectrophotometer. The molecular level interaction of HEMA with atelocollagen, which was used as a collagen model, was investigated by STD-NMR spectroscopy.
The HEMA concentration in the suspension did not change, indicating that HEMA did not bind to dentin collagen. This was confirmed by STD-NMR; when the atelocollagen resonance was saturated, no saturation was propagated to HEMA and no STD signals were detected.
The HEMA protons were not near the atelocollagen surface, indicating HEMA did not interact with atelocollagen. The collagen fibrils may be surrounded by water molecules in dentin/bond interfaces, which prevent the direct HEMA binding interaction.
甲基丙烯酸羟乙酯（HEMA）在湿牙本质中扩散，并在牙本质预处理和粘结过程中促进粘结。我们通过使用饱和转移差（STD）核磁共振技术研究了HEMA与胶原蛋白模型之间的分子水平相互作用。
通过将脱矿的人牙本质粉末悬浮在4mM HEMA溶液中1小时，并在分光光度计上测量HEMA浓度的降低，初步研究了HEMA与胶原蛋白的结合。通过STD-核磁共振光谱研究了HEMA与用作胶原蛋白模型的去端肽胶原蛋白的分子水平相互作用。
悬浮液中的HEMA浓度没有变化，表明HEMA没有与牙本质胶原蛋白结合。这通过STD-核磁共振得到证实；当去端肽胶原蛋白共振饱和时，没有饱和传递到HEMA，也没有检测到STD信号。
HEMA质子不在去端肽胶原蛋白表面附近，表明HEMA没有与去端肽胶原蛋白相互作用。牙本质/粘结界面中的胶原纤维可能被水分子包围，这阻止了HEMA的直接结合相互作用。
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