Andreasen J R, Glisson J R, Goodwin M A, Resurreccion R S, Villegas P, Brown J
Department of Avian Medicine, College of Veterinary Medicine, University of Georgia, Athens 30605.
Avian Dis. 1989 Jul-Sep;33(3):516-23.
Broiler chickens were vaccinated at 18 days of age against infectious laryngotracheitis (ILT) using chicken-embryo-origin (CEO) and tissue-culture-origin (TCO) vaccines, each vaccine given either by drinking water, spray, or eyedrop. Controls were not vaccinated. The broilers were challenged 3 weeks later with virulent ILT virus (USDA challenge strain). Serum samples taken before challenge were analyzed by a virus neutralization (VN) test to determine titers due to vaccination. Both vaccines, regardless of route of administration, produced low VN titers, geometric mean titer (GMT) being less than 4.0 in all vaccinated groups. When administered by the same route, the CEO vaccine produced higher titers than the TCO vaccine. Titers following drinking-water or eyedrop administration of vaccines were higher than titers following spray vaccination. There was an inverse relationship between pre-challenge VN titers of groups of birds and the percentage of birds in the groups dying from ILT virus challenge. The drinking-water route of vaccination provided the most protection, while the spray provided the least.
18日龄的肉鸡使用鸡胚源（CEO）和组织培养源（TCO）疫苗接种传染性喉气管炎（ILT）疫苗，每种疫苗通过饮水、喷雾或滴眼方式接种。对照组未接种疫苗。3周后用强毒ILT病毒（美国农业部攻毒株）对肉鸡进行攻毒。攻毒前采集的血清样本通过病毒中和（VN）试验进行分析，以确定疫苗接种后的滴度。两种疫苗，无论接种途径如何，产生的VN滴度都很低，所有接种组的几何平均滴度（GMT）均低于4.0。当通过相同途径接种时，CEO疫苗产生的滴度高于TCO疫苗。通过饮水或滴眼接种疫苗后的滴度高于喷雾接种后的滴度。鸡群攻毒前的VN滴度与鸡群中死于ILT病毒攻毒的鸡的百分比之间呈负相关。饮水接种途径提供的保护最多，而喷雾接种提供的保护最少。
