Department of Pharmaceutical Sciences, Biomanufacturing Research Institute and Technology Enterprise (BRITE), North Carolina Central University, Durham, NC, USA.
Department of Pharmaceutical Sciences, Biomanufacturing Research Institute and Technology Enterprise (BRITE), North Carolina Central University, Durham, NC, USA.
J Environ Sci (China). 2014 Dec 1;26(12):2526-34. doi: 10.1016/j.jes.2014.04.010. Epub 2014 Nov 4.
Multi-walled carbon nanotube (MWCNT) sheet was fabricated from a drawable MWCNT forest and then deposited on poly(methyl methacrylate) film. The film was further coated with a natural antimicrobial peptide nisin. We studied the effects of nisin coating on the attachment of Bacillus anthracis spores, the germination of attached spores, and the subsequent biofilm formation from attached spores. It was found that the strong adsorptivity and the super hydrophobicity of MWCNTs provided an ideal platform for nisin coating. Nisin coating on MWCNT sheets decreased surface hydrophobicity, reduced spore attachment, and reduced the germination of attached spores by 3.5 fold, and further inhibited the subsequent biofilm formation by 94.6% compared to that on uncoated MWCNT sheet. Nisin also changed the morphology of vegetative cells in the formed biofilm. The results of this study demonstrated that the anti-adhesion and antimicrobial effect of nisin in combination with the physical properties of carbon nanotubes had the potential in producing effective anti-biofilm formation surfaces.
多壁碳纳米管 (MWCNT) 片由可拉伸的 MWCNT 森林制成，然后沉积在聚甲基丙烯酸甲酯薄膜上。该薄膜进一步涂覆有天然抗菌肽乳链菌肽。我们研究了乳链菌肽涂层对炭疽芽孢杆菌孢子附着、附着孢子发芽以及随后由附着孢子形成生物膜的影响。结果发现，MWCNTs 的强吸附性和超疏水性为乳链菌肽涂层提供了理想的平台。MWCNT 片上的乳链菌肽涂层降低了表面疏水性，减少了孢子附着，并使附着孢子的发芽减少了 3.5 倍，与未涂层的 MWCNT 片相比，进一步抑制了随后生物膜形成的 94.6%。乳链菌肽还改变了形成生物膜中营养细胞的形态。本研究结果表明，乳链菌肽的抗粘附和抗菌作用与碳纳米管的物理性质相结合，有可能产生有效的抗生物膜形成表面。
