Jung Ha-Young, Seo Dong-Won, Hong Chung-Oui, Kim Ji-Yeon, Yang Sung-Yong, Lee Kwang-Won
Department of Food Bioscience and Technology, College of Life Science & Biotechnology, Korea University, Seoul 136-713, Republic of Korea.
Department of Food Bioscience and Technology, College of Life Science & Biotechnology, Korea University, Seoul 136-713, Republic of Korea; Food Analysis Center, Korea Food Research Institute, 516, Baekhyeon, Bundang, Seongnam, Gyeonggi 463-746, Republic of Korea.
Environ Toxicol Pharmacol. 2015 Jan;39(1):125-36. doi: 10.1016/j.etap.2014.11.012. Epub 2014 Nov 30.
Cadmium (Cd), an environmental and industrial pollutant, generates free radicals responsible for oxidative stress. Cd can also lead to various renal toxic damage such as the proximal tubules and glomerulus dysfunction. Plantamajoside (PMS), a major compound of Plantago asiatica (PA), was reported to have the antioxidant effects. In this study, we investigated the protective effects of PMS on Cd-induced renal damage in the NRK-52E cell and rat kidney tissue. Cd exposure increased the ROS generation, lipid peroxidation, serum biochemical values of renal damage, and mRNA and protein expressions of KIM-1 in vitro and in vivo. The significant reduction in glutathione (GSH)/glutathione disulfide (GSSG) ratio and activities of antioxidant enzymes were also observed in the rats treated with Cd. PMS significantly decreased the ROS generation and lipid peroxidation, thus enhancing GSH/GSSG ratio, antioxidant enzyme activities in the cells and rats, and improved histochemical appearances, indicating that PMS has protective activities against Cd-induced renal injury.
镉（Cd）作为一种环境和工业污染物，会产生导致氧化应激的自由基。Cd还可导致各种肾脏毒性损伤，如近端小管和肾小球功能障碍。大车前苷（PMS）是车前草（PA）的主要成分，据报道具有抗氧化作用。在本研究中，我们研究了PMS对NRK-52E细胞和大鼠肾组织中Cd诱导的肾损伤的保护作用。Cd暴露在体外和体内均增加了活性氧（ROS）生成、脂质过氧化、肾损伤的血清生化值以及肾损伤分子-1（KIM-1）的mRNA和蛋白表达。在用Cd处理的大鼠中还观察到谷胱甘肽（GSH）/谷胱甘肽二硫化物（GSSG）比值和抗氧化酶活性显著降低。PMS显著降低了ROS生成和脂质过氧化，从而提高了细胞和大鼠中的GSH/GSSG比值、抗氧化酶活性，并改善了组织化学外观，表明PMS对Cd诱导的肾损伤具有保护作用。
