含有合成同构核苷替代物的小干扰RNA寡核苷酸的细胞活性
Cellular activity of siRNA oligonucleotides containing synthetic isomorphic nucleoside surrogates.
作者信息
Shin Dongwon, Lönn Peter, Dowdy Steven F, Tor Yitzhak
机构信息
Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla CA 92093-0358, USA.
出版信息
Chem Commun (Camb). 2015 Jan 31;51(9):1662-5. doi: 10.1039/c4cc08809c.
Abstract
Singly and multiply modified synthetic siRNA oligonucleotides, containing isomorphic surrogate nucleobases, show high interference potency in cell culture, suggesting the highly isomorphic RNA alphabet, based on a thieno[3,4-d]-pyrimidine core, is tolerated well by the cellular silencing machinery.
摘要
含有同构替代核碱基的单修饰和多修饰合成小干扰RNA（siRNA）寡核苷酸在细胞培养中显示出高干扰效力，这表明基于噻吩并[3,4-d]嘧啶核心的高度同构RNA字母表能被细胞沉默机制很好地耐受。
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