Yue Y Z, Sun J, Huang X, Peng H, Liu G F, Hu H R
Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, Hubei Province, China.
Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, Hubei Province, China
Genet Mol Res. 2014 Nov 27;13(4):9889-98. doi: 10.4238/2014.November.27.17.
In Petunia x hybrida 'Fantasy Red', a leucine-rich repeat (LRR) gene referred to as PhLRR, was identified in a flower bud cDNA library. The open reading frame sequence of PhLRR was 1251 bp, encoding a putative 46.2-kDa protein of 416 amino acids. The PhLRR protein showed high similarity to members of polygalacturonase inhibitor proteins (PGIPs), contained 11 conserved LRR domains, and was an extracellular localization protein. Phylogenetic analysis showed that PhLRR belonged to the same PGIPs subfamily as SHY, indicating that PhLRR may be involved in the development of pollen-like SHY. Expression analysis revealed that PhLRR was abundantly expressed during early stages of flower bud and anther development, while it was not detected in any other examined organs, such as sepals, petals, pistils, roots, stems, leaves, or open flowers. Furthermore, many cis-acting elements (such as AGAAA and GTGA) related to anther-specific gene expression were identified in the PhLRR gene promoter region, indicating that the promoter is also anther-specific. These results suggested that PhLRR is a novel anther-specific gene that may be essential for the early development of anthers.
在矮牵牛杂交种‘幻想红’中，一个名为PhLRR的富含亮氨酸重复序列（LRR）基因在花芽cDNA文库中被鉴定出来。PhLRR的开放阅读框序列为1251 bp，编码一个由416个氨基酸组成的推定的46.2 kDa蛋白质。PhLRR蛋白与多聚半乳糖醛酸酶抑制蛋白（PGIPs）成员具有高度相似性，包含11个保守的LRR结构域，并且是一种细胞外定位蛋白。系统发育分析表明，PhLRR与SHY属于同一PGIPs亚家族，这表明PhLRR可能参与了类似花粉的SHY的发育。表达分析显示，PhLRR在花芽和花药发育的早期大量表达，而在任何其他检测的器官中均未检测到，如萼片、花瓣、雌蕊、根、茎、叶或开放的花朵。此外，在PhLRR基因启动子区域鉴定出许多与花药特异性基因表达相关的顺式作用元件（如AGAAA和GTGA），这表明该启动子也是花药特异性的。这些结果表明，PhLRR是一个新的花药特异性基因，可能对花药的早期发育至关重要。
