低电压和高频电穿孔可增加内皮细胞通透性。
The endothelial permeability increased by low voltage and high frequency electroporation.
作者信息
Shankayi Zeinab, Pourmirjafari Firoozabadi Seyed Mohammad, Zohair Saraf Hassan
机构信息
Department of Medical Physics, faculty of medical science, Tarbiat Modares University, Tehran, Iran.
Department of Immunology, faculty of medical science, Tarbiat Modares University, Tehran, Iran.
出版信息
J Biomed Phys Eng. 2013 Sep 17;3(3):87-92. eCollection 2013 Sep.
UNLABELLED
Propose: Standard electroporation and electrochemotherapy caused the endothelial cell permeability and reduction in tumor blood flow. The effects of low voltage and high frequency electroporation on the endothelial cells permeability and viability were expected. Therefore, the propose of present study was to evaluate the effect of electroporation with bleomycin or alone on viability and permeabilization of human Embryo microvascular endothelial (HUVEC) cell line.
MATERIAL AND METHODS
The HUVEC cells were exposed to 4000 electric pulsed with 100µs duration, 50-150 v/cm with increment of 10 v/cm in 4, 5 and 6 kHz frequency (33 experimental groups) and then uptake and viability reduction was measured in each group.
RESULTS
The results of this study demonstrated that electric pulses alone reduce the cell viability and with bleomycin significantly increases the toxicity to endothelial cells.
CONCLUSION
Our data indicated that low voltage, high frequency ECT is highly cytotoxic for a HUVEC cell offers a possible effect of antivascular actions of this kind of electrochemotherapy.
未标注
提出：标准电穿孔和电化学疗法会导致内皮细胞通透性增加和肿瘤血流减少。预期低电压高频电穿孔对内皮细胞通透性和活力的影响。因此，本研究的目的是评估博来霉素电穿孔或单独电穿孔对人胚胎微血管内皮（HUVEC）细胞系活力和通透性的影响。
材料与方法
将HUVEC细胞暴露于持续时间为100µs、电压为50 - 150 v/cm、以10 v/cm递增、频率分别为4、5和6 kHz的4000个电脉冲下（共33个实验组），然后测量每组细胞的摄取量和活力降低情况。
结果
本研究结果表明，单独电脉冲会降低细胞活力，而与博来霉素联合使用会显著增加对内皮细胞的毒性。
结论
我们的数据表明，低电压高频电穿孔对HUVEC细胞具有高度细胞毒性，为这种电化学疗法的抗血管作用提供了一种可能的效果。
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