来自亚马逊河流域的三种电鱼（无背鳍电鳗科，裸背电鳗目）的染色体多样性。
Chromosomal diversity in three species of electric fish (Apteronotidae, Gymnotiformes) from the Amazon Basin.
作者信息
Silva Fernando Henrique Ramos, Pieczarka Julio Cesar, Cardoso Adauto Lima, da Silva Patrícia Corrêa, de Oliveira Jonas Alves, Nagamachi Cleusa Yoshiko
机构信息
Instituto de Ciências Biológicas , Universidade Federal do Pará , Belém, PA , Brazil .
Instituto de Desenvolvimento Sustentável Mamirauá , Tefé, AM , Brazil .
出版信息
Genet Mol Biol. 2014 Oct;37(4):638-45. doi: 10.1590/S1415-47572014005000018. Epub 2014 Nov 3.
Cytogenetic studies were carried out on samples of Parapteronotus hasemani, Sternarchogiton preto and Sternarchorhamphus muelleri (Apteronotidae, Gymnotiformes) from the Amazon basin. The first two species exhibited both a 2n = 52 karyotype, but differed in their karyotypic formulae, distribution of constitutive heterochromatin, and chromosomal location of the NOR. The third species, Sternarchorhamphus muelleri, was found to have a 2n = 32 karyotype. In all three species the DAPI and chromomycin A3 staining results were consistent with the C-banding results and nucleolar organizer region (NOR) localization. The 18S rDNA probe confirmed that there was only one pair of ribosomal DNA cistron bearers per species. The telomeric probe did not reveal interstitial telomeric sequences (ITS). The karyotypic differences among these species can be used for taxonomic identification. These data will be useful in future studies of these fishes and help understanding the phylogenetic relationships and chromosomal evolution of the Apteronotidae.
对来自亚马逊盆地的哈氏副电鳗（Parapteronotus hasemani）、普氏电臀鳗（Sternarchogiton preto）和穆氏电吻电鳗（Sternarchorhamphus muelleri）（裸背电鳗科，电鳗目）样本进行了细胞遗传学研究。前两个物种均呈现2n = 52的核型，但它们的核型公式、组成型异染色质分布以及核仁组织区（NOR）的染色体定位有所不同。第三个物种，穆氏电吻电鳗，被发现具有2n = 32的核型。在所有三个物种中，4,6-二脒基-2-苯基吲哚（DAPI）和放线菌素A3染色结果与C带结果以及核仁组织区（NOR）定位一致。18S核糖体DNA（rDNA）探针证实每个物种仅有一对核糖体DNA顺反子携带者。端粒探针未揭示间质端粒序列（ITS）。这些物种之间的核型差异可用于分类鉴定。这些数据将有助于未来对这些鱼类的研究，并有助于理解裸背电鳗科的系统发育关系和染色体进化。
Zotero 插件