Sharma Aanchal, Sahni Priya, Nayak Meghanand T, Singhvi Abhishek, Kumar Rajesh
J Exp Ther Oncol. 2014;10(4):317-23.
Oral Submucous Fibrosis (OSF) is a well-recognized, potentially malignant disorder causing generalized fibrosis of the submucosal oral soft tissues. Though this disease is believed to be multi-factorial, areca nut chewing has emerged as the most important causative factor for OSF. Areca nut is known to have high levels of copper, which is believed to cause lysyl oxidase associated fibrosis.
To evaluate the pattern of copper in buccal mucosal cells of OSF patients, areca nut chewers and normal healthy individuals and to elicit the etiology of copper in OSF.
Patients were divided into three groups each comprising of 20 individuals- Healthy individuals (Group I); areca nut chewers without OSF (Group II); histopathologically confirmed OSF (Group Ill). The cytological smears made from each patient were stained with rhodanine stain for copper and evaluated for the qualitative and quantitative parameters of copper by using specific grading criteria.
Quantitative estimation of copper content showed a marked variation in the mean values. Mean value of group I was 0.11 ± 0.39; group II was 1.09 ± 0.81 and group III was 2.34 ± 0.74 (p<0.001). Mean values for qualitative estimation of copper were - 0.01 ± 0.36 for group I, 1.08 ± 0.82 for group II and 2.39 ± 0.72 for group III (p<0.001). Chi square analysis was used to assess the percentage distribution of copper granules. This revealed that the colour intensity and the number of granules were seen to maximum in OSF patients, areca nut chewers without OSF having intermediated values and normal healthy individuals having the least values.
An evident increase in the copper staining in group III individuals as compared to group I and group II was well appreciated. Increased copper levels in the local environment of the oral cavity indicates its role in lysyl oxidase associated submucosal fibrosis.
口腔黏膜下纤维化（OSF）是一种公认的、具有潜在恶性的疾病，可导致口腔黏膜下软组织的广泛性纤维化。尽管这种疾病被认为是多因素的，但嚼槟榔已成为OSF最重要的致病因素。已知槟榔中铜含量很高，据信这会导致赖氨酰氧化酶相关的纤维化。
评估OSF患者、嚼槟榔者和正常健康个体颊黏膜细胞中的铜模式，并探讨OSF中铜的病因。
将患者分为三组，每组20人——健康个体（第一组）；无OSF的嚼槟榔者（第二组）；经组织病理学确诊的OSF患者（第三组）。对每位患者制作的细胞学涂片用罗丹宁染色法进行铜染色，并使用特定的分级标准评估铜的定性和定量参数。
铜含量的定量估计显示平均值有显著差异。第一组的平均值为0.11±0.39；第二组为1.09±0.81，第三组为2.34±0.74（p<0.001）。铜定性估计的平均值——第一组为-0.01±0.36，第二组为1.08±0.82，第三组为2.39±0.72（p<0.001）。采用卡方分析评估铜颗粒的百分比分布。结果显示，OSF患者中铜颗粒的颜色强度和数量最多，无OSF的嚼槟榔者的值居中，正常健康个体的值最少。
与第一组和第二组相比，第三组个体的铜染色明显增加，这一点很明显。口腔局部环境中铜水平的升高表明其在赖氨酰氧化酶相关的黏膜下纤维化中起作用。
