Cátedra de Fisiología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, IQUIMEFA-CONICET, Junín 956, C1113AAD, Ciudad Autónoma De Buenos Aires, Argentina.
Cátedra de Química General e Inorgánica, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, C1113AAD, Ciudad Autónoma De Buenos Aires, Argentina.
Eur J Nutr. 2016 Feb;55(1):33-43. doi: 10.1007/s00394-014-0820-y. Epub 2014 Dec 18.
During the postnatal stage, cardiovascular nitric oxide (NO) system and caveolins (cav) may be regulated differentially in response to hypovolemic state induced by water restriction. Our aim was to examine the effects of water restriction on NO synthases (NOS) and cav in the atria, ventricle and aorta of growing rats.
Male Sprague-Dawley rats aged 25 and 50 days were divided into (n = 15): WR: water restriction 3 days; WAL: water ad libitum 3 days. Systolic blood pressure, NOS activity and NOS/cav protein levels were measured.
Dehydration induced a larger increase in SBP in WR25 group. Ventricular NOS activity, endothelial NOS (eNOS) and neuronal isoform (nNOS) of WR25 pups were increased, and both cav were decreased. In the WR50 group, NOS activity remained unchanged. In the atria, NOS activity, eNOS and nNOS decreased in WR25 associated with increased cav-1; in the WR50 group, NOS activity was increased without changes in NOS isoforms. In the aorta of WR25, NOS activity and inducible NOS (iNOS) were decreased; NOS activity was unchanged in WR50, despite the decreased levels of eNOS and increased iNOS, cav-1 and cav-3.
NO system adjustments in cardiovascular system under osmotic stress in vivo depend on postnatal age, being eNOS and nNOS, the isoforms that determine NOS activity in cardiac tissue in 25-day-old pups. Changes in cav abundance during hypovolemic state may contribute to age-related NO production.
在产后阶段，心血管一氧化氮（NO）系统和 caveolins（cav）可能会因水限制引起的低血容量状态而产生差异调节。我们的目的是研究水限制对生长大鼠心房、心室和主动脉中 NO 合酶（NOS）和 cav 的影响。
25 日龄和 50 日龄雄性 Sprague-Dawley 大鼠分为（n = 15）：WR：水限制 3 天；WAL：自由饮水 3 天。测量收缩压、NOS 活性和 NOS/cav 蛋白水平。
脱水导致 WR25 组的 SBP 升高幅度更大。WR25 幼鼠的心室 NOS 活性、内皮型一氧化氮合酶（eNOS）和神经元同工型（nNOS）增加，两种 cav 均减少。在 WR50 组中，NOS 活性保持不变。在心房中，WR25 幼鼠的 NOS 活性、eNOS 和 nNOS 降低，与 cav-1 增加有关；在 WR50 组中，NOS 活性增加，NOS 同工型无变化。在 WR25 的主动脉中，NOS 活性和诱导型 NOS（iNOS）降低；尽管 eNOS 水平降低和 iNOS 增加，但 WR50 中的 NOS 活性保持不变，cav-1 和 cav-3 增加。
体内渗透压应激下心血管系统 NO 系统的调节取决于出生后年龄，eNOS 和 nNOS 是决定 25 日龄幼鼠心肌组织 NOS 活性的同工型。低血容量状态下 cav 丰度的变化可能与年龄相关的 NO 产生有关。
