Ghahremani Enayat, Mardani Mahnaz, Rezapour Sadegh
Department of Microbiology, Science and Research Branch, Islamic Azad University, Arak, Iran,
Appl Biochem Biotechnol. 2015 Mar;175(5):2516-27. doi: 10.1007/s12010-014-1434-9. Epub 2014 Dec 19.
Lactic acid bacteria (LAB) with proteolitic activity are used as aromatic and antibacterial substances, cholesterol reduces, bile salt hydrolyses, and probiotic. The aims of this project were to isolate and identify natural LAB flora involved in traditional fermentation in cheeses of Khoramabad city and also to survey their probiotic potential. In order to achieve this goal, LAB were isolated and characterized using phenotypic and genotypic methods (PCR-sequencing); in the next stage, they were analyzed lowering cholesterol medium, hydrolysis of the bile, resistance to bile-resistant PH acidic stomach. At the end of the study, 88 cocci and 3 bacill were found: 58 Enterococcus faecium, 16 Enterococcus hirae, 5 Lactococcus lactis, 3 Lactobacillus plantarum, and 9 undetermined. The probiotic results of the bacteria had effects on the reduction of cholesterol, resistance to stomach acid, had relative antibacterial effects, and some strains had effects on hydrolyzing the bile. For further identification, the PCR method and the application of 16s-DNA-ITS genes and its sequencing were found useful. This study showed that lactic acid bacteria in the traditional cheese of the Khorramabad city have relative probiotic effect and that these lactic acid bacteria in fermented milk are suitable.
具有蛋白水解活性的乳酸菌用作芳香和抗菌物质、降低胆固醇、进行胆汁盐水解以及作为益生菌。本项目的目的是分离和鉴定参与霍拉马巴德市奶酪传统发酵的天然乳酸菌菌群，并研究它们的益生菌潜力。为实现这一目标，采用表型和基因型方法（PCR测序）对乳酸菌进行分离和鉴定；在下一阶段，对它们在降低胆固醇培养基、胆汁水解、耐胆汁耐酸性胃方面进行分析。研究结束时，发现了88株球菌和3株杆菌：58株粪肠球菌、16株希氏肠球菌、5株乳酸乳球菌、3株植物乳杆菌和9株未确定的菌株。这些细菌的益生菌结果对降低胆固醇、耐胃酸、具有相对抗菌作用，并且一些菌株对胆汁水解有作用。为进一步鉴定，发现PCR方法以及16s - DNA - ITS基因的应用及其测序是有用的。本研究表明，霍拉马巴德市传统奶酪中的乳酸菌具有相对益生菌作用，并且这些发酵乳中的乳酸菌是合适的。
