Bauer Jan, Lassmann Hans
Department of Neuroimmunology, Center for Brain Research, Medical University of Vienna, Spitalgasse 4, 1090, Vienna, Austria.
Methods Mol Biol. 2016;1304:211-29. doi: 10.1007/7651_2014_151.
Immunohistochemical techniques (IHC) and in situ hybridization (ISH) are widely used techniques to study the expression of proteins and messenger RNAs in tissues and are extremely important to confirm and interpret biochemical and molecular results from the same tissues. Investigation of human brain by IHC and ISH therefore still plays an important role in the elucidation of pathogenetic mechanisms in diseases such as multiple sclerosis. In this review we describe the processing of human brain tissues as well as basic and advanced immunohistochemical staining and ISH techniques used for neuropathological analysis of such pathological brains.
免疫组织化学技术（IHC）和原位杂交（ISH）是广泛用于研究组织中蛋白质和信使核糖核酸表达的技术，对于确认和解释来自同一组织的生化和分子结果极为重要。因此，通过免疫组织化学技术和原位杂交对人脑进行研究，在阐明诸如多发性硬化症等疾病的发病机制方面仍发挥着重要作用。在本综述中，我们描述了人脑组织的处理方法，以及用于此类病理性脑的神经病理学分析的基础和先进免疫组织化学染色及原位杂交技术。
