Ivanova T N, Ivanov A I, Korovkin B F, Losev N I
Biull Eksp Biol Med. 1989 Jul;108(7):30-2.
The acid phosphatase and cathepsin D activities and cAMP and cGMP levels in isolated perfused rat heart were investigated during various periods of ischaemic myocardial injury and postischaemic reperfusion. The effect of phosphodiesterase inhibitor--caffeine was also studied. Free acid hydrolases activities and cyclic nucleotide content were increased under 40 and 60 min ischemia and 20 min postischaemic reperfusion. Addition of 50 microM caffeine to perfusion solution after 30 min of ischaemia resulted in increase of cAMP level, cAMP/cGMP ratio, lysosomal bound activities of acid hydrolase and decrease of free acid hydrolase activities. The obtained results suggested that defect in cAMP synthesis might be present in lysosomal membranes labilization in cardiomyocytes injured during ischaemic conditions. Addition of such agents, as caffeine, which increased heart cAMP level, may be effective in lysosomal membranes stabilization under reversible heart ischaemia and reperfusion.
在缺血性心肌损伤和缺血后再灌注的不同阶段，对离体灌注大鼠心脏中的酸性磷酸酶和组织蛋白酶D活性以及环磷酸腺苷（cAMP）和环磷酸鸟苷（cGMP）水平进行了研究。还研究了磷酸二酯酶抑制剂——咖啡因的作用。在缺血40和60分钟以及缺血后再灌注20分钟时，游离酸性水解酶活性和环核苷酸含量增加。缺血30分钟后向灌注液中添加50微摩尔咖啡因导致cAMP水平、cAMP/cGMP比值升高，酸性水解酶的溶酶体结合活性增加，游离酸性水解酶活性降低。所得结果表明，在缺血条件下受损的心肌细胞中，溶酶体膜不稳定可能存在cAMP合成缺陷。添加如咖啡因等能提高心脏cAMP水平的药物，可能对可逆性心脏缺血和再灌注时的溶酶体膜稳定有效。
