Li Yubo, Zang Dawei
Capital Medical University, Beijing, 100069, China.
Department of Neurology, Tianjin First Center Hospital, Tianjin Medical University, Tianjin, 300192, China.
PLoS One. 2014 Dec 26;9(12):e116031. doi: 10.1371/journal.pone.0116031. eCollection 2014.
To explore whether LIF could promote the proliferation of neural precursor cells (NPCs) and to analyze the correlation between increased NPCs and FluoroGold (FG) labeled neurons in mice after spinal cord injury (SCI).
Motor behavior was assessed using Rotarod and Platform Hang tests; neurons in the corticospinal and rubrospinal systems were labeled with FG, NPCs were immustained with nestin-FITC conjugate. The numbers of FG-labeled neurons and NPCs were estimated, and the correlation between FG-labeled neurons and NPCs was assessed.
Mice in the SCI group showed negligible recovery of locomotor behavior; in contrast, mice in the LIF group showed a statically significant improvement. Both FG-labeled neurons and NPCs were significantly increased in the LIF group compared to the SCI group, and this increase in FG-labeled neurons and NPCs showed a clear association above the lesion level.
LIF could promote locomotive behaviors in mice post-SCI by encouraging the proliferation of NPCs; LIF may in fact be a potential cytokine for the induction of NPCs post-SCI.
探讨白血病抑制因子（LIF）是否能促进神经前体细胞（NPCs）的增殖，并分析脊髓损伤（SCI）后小鼠体内NPCs增加与荧光金（FG）标记神经元之间的相关性。
采用转棒试验和平台悬挂试验评估运动行为；用FG标记皮质脊髓和红核脊髓系统中的神经元，用巢蛋白-异硫氰酸荧光素（nestin-FITC）偶联物对NPCs进行免疫染色。估计FG标记神经元和NPCs的数量，并评估FG标记神经元与NPCs之间的相关性。
SCI组小鼠的运动行为恢复可忽略不计；相比之下，LIF组小鼠的运动行为有显著改善。与SCI组相比，LIF组中FG标记的神经元和NPCs均显著增加，且损伤水平以上FG标记的神经元和NPCs的增加呈现明显的相关性。
LIF可通过促进NPCs的增殖来促进SCI后小鼠的运动行为；事实上，LIF可能是SCI后诱导NPCs的一种潜在细胞因子。
