Yamagata S, Tsuchida S, Yamamoto K, Kawamura N, Nakamura T, Ueki S, Sugawa T
Department of Obstetrics and Gynecology, Osaka City University Medical School.
Nihon Sanka Fujinka Gakkai Zasshi. 1989 Sep;41(9):1334-40.
Routinely paraffin-embedded sections of dysplasia, carcinoma in situ (CIS) and invasive (squamous) carcinoma of the cervix were studied to determine the participation of human papilloma virus (HPV) in these tissues. Morphological observation (1,059 cases) revealed condylomatous changes to reach 54% in dysplasia, 25% in CIS and 25% in invasive carcinoma. Condylomatous changes were also found to be 25 to 40% in the non-cancerous epithelia adjacent to in situ or invasive carcinomas. The immuno-peroxidase-PAP-method using anti-HPV serum was applied to 98 selected sections in which condylomatous changes were morphologically observed. HPV antigens were found to reach 56% in dysplasia, 42% in CIS and 35% in invasive carcinoma, and this result suggested that the morphologically observed condylomatous changes did not always coincide with virus maturation in the infected cells. By means of the in situ hybridization technique, HPV type-6, -11, -16 and -18 DNAs were all detected in dysplasia sections, whereas HPV type-16 DNA was demonstrated distinctively at a high rate among in situ and invasive carcinomas.
对宫颈发育异常、原位癌（CIS）及浸润性（鳞状）癌的常规石蜡包埋切片进行研究，以确定人乳头瘤病毒（HPV）在这些组织中的参与情况。形态学观察（1059例）显示，湿疣样改变在发育异常中占54%，在原位癌中占25%，在浸润性癌中占25%。在原位癌或浸润性癌相邻的非癌上皮中也发现湿疣样改变占25%至40%。对98个经形态学观察有湿疣样改变的选定切片应用抗HPV血清免疫过氧化物酶 - PAP法。发现HPV抗原在发育异常中占56%，在原位癌中占42%，在浸润性癌中占35%，这一结果表明形态学观察到的湿疣样改变并不总是与受感染细胞中的病毒成熟一致。通过原位杂交技术，在发育异常切片中均检测到HPV 6型、11型、16型和18型DNA，而在原位癌和浸润性癌中，HPV 16型DNA以高比例显著显示。
