免疫性血小板减少症患者树突状细胞中 CD205 与 CD80/CD86 的失衡。
Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia.
机构信息
Department of Hematology, Qilu Hospital, Shandong University, Jinan, China.
Department of Hematology, Qilu Hospital, Shandong University, Jinan, China.
出版信息
Thromb Res. 2015 Feb;135(2):352-61. doi: 10.1016/j.thromres.2014.11.042. Epub 2014 Dec 19.
INTRODUCTION
CD205(DEC-205), a tolerance-associated receptor, is a member of the macrophage mannose receptor family of C-type lectin receptors. Antigen uptake via CD205 induces regulatory T cells, thereby regulating peripheral immune tolerance. However, the contribution of CD205 to autoimmune diseases has not been elucidated. Immune thrombocytopenia (ITP) is an autoimmune disorder characterized by overdestruction of platelets. A previous study by the present authors found that CD205 expression in dendritic cells (DCs) was upregulated during induction of immune tolerance in patients with ITP.
METHODS
CD205 expression in monocyte-derived DCs and spleens from patients with ITP was analysed prior to and after high-dose dexamethasone (HD-DXM) treatment. Expression of CD80, CD86 and HLA-DR was also analysed in order to identify and define the maturation status of the DCs more precisely.
RESULTS
In patients with ITP, CD205 expression was found to be significantly decreased in DCs, and rare or absent in the border region of the spleen. However, the expression of CD80 and CD86 was increased in both monocyte-derived DCs and spleens in patients with ITP compared with controls. HD-DXM treatment may upregulate CD205 expression and downregulate CD80/CD86 expression, then rebalance the expression of CD205 and CD80/CD86 in DCs in patients with ITP.
CONCLUSION
Imbalance between CD205 and CD80/CD86 may contribute to the development of ITP. Therapies that aim to restore the balance between CD205 and CD80/CD86 may help to re-establish tolerance in patients with ITP.
简介
CD205（DEC-205）是一种与耐受相关的受体，是巨噬细胞甘露糖受体家族 C 型凝集素受体的成员。通过 CD205 摄取抗原可诱导调节性 T 细胞，从而调节外周免疫耐受。然而，CD205 对自身免疫性疾病的贡献尚未阐明。免疫性血小板减少症（ITP）是一种自身免疫性疾病，其特征是血小板过度破坏。本研究作者之前的一项研究发现，ITP 患者诱导免疫耐受期间，树突状细胞（DC）中 CD205 的表达上调。
方法
在接受大剂量地塞米松（HD-DXM）治疗前后，分析 ITP 患者单核细胞来源的 DC 和脾脏中 CD205 的表达。还分析了 CD80、CD86 和 HLA-DR 的表达，以更准确地识别和定义 DC 的成熟状态。
结果
在 ITP 患者中，发现 DC 中 CD205 的表达显著降低，在脾脏的边缘区域很少或不存在。然而，与对照组相比，ITP 患者的单核细胞来源的 DC 和脾脏中 CD80 和 CD86 的表达增加。HD-DXM 治疗可能上调 CD205 的表达并下调 CD80/CD86 的表达，从而使 ITP 患者的 DC 中 CD205 和 CD80/CD86 的表达重新平衡。
结论
CD205 和 CD80/CD86 之间的失衡可能导致 ITP 的发生。旨在恢复 CD205 和 CD80/CD86 之间平衡的治疗方法可能有助于重新建立 ITP 患者的耐受。
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