[布宜诺斯艾利斯蚊子中圣路易斯脑炎病毒的分子检测]

[Molecular detection of Saint Louis encephalitis virus in mosquitoes in Buenos Aires].

作者信息

Beltrán Fernando J, Bechara Yamila I, Guido Guillermo G, Cicuttin Gabriel L, Beaudoin Juan B, Gury Dohmen Federico E

机构信息

área de Zoonosis Virales, Sección Serología y Pruebas Biológicas, Instituto de Zoonosis Luis Pasteur (SSPB-IZLP), Buenos Aires, Argentina. E-mail:

出版信息

Medicina (B Aires). 2014;74(6):433-6.

PMID:25555001

Abstract

During March 2013 a population of eared doves (Zenaida auriculata) was established in the center of City of Buenos Aires. Considering the role of these birds as host competent for Saint Louis encephalitis virus (SLEV), a CDC light trap was put in place to perform entomologic surveillance. During this month 5 pools of mosquitoes (n = 48) were collected and taxonomically determined. Three of them were classified as Culex pipiens (n = 10) and the other two were Culex spp. (n = 38). In this case, the mosquitoes species could not be determined due to that individuals were damaged. One of the Culex spp. pool was found to be positive for Saint Louis encephalitis virus by molecular techniques. This was then sequenced and classified as genotype III.

摘要

2013年3月期间，布宜诺斯艾利斯市中心引入了一群斑颊哀鸽（Zenaida auriculata）。鉴于这些鸟类作为圣路易斯脑炎病毒（SLEV）易感宿主的作用，设置了一个疾控中心轻型诱蚊灯进行昆虫学监测。在这个月里，收集了5组蚊子（n = 48）并进行了分类鉴定。其中3组被归类为致倦库蚊（n = 10），另外两组为库蚊属（n = 38）。在这种情况下，由于个体受损，无法确定蚊子的种类。通过分子技术发现其中一组库蚊属蚊子对圣路易斯脑炎病毒呈阳性。随后对其进行测序并归类为III型基因型。