Department of Analytical Chemistry, University of Pannonia, Egyetem utca 10, H-8200 Veszprém, Hungary.
MTA-PTE Molecular Interactions in Separation Science Research Group, Ifjúság útja 6, H-7624 Pécs, Hungary.
J Chromatogr A. 2015 Jan 23;1378:32-6. doi: 10.1016/j.chroma.2014.12.001. Epub 2014 Dec 9.
An algorithm was developed for the minimization of consumption of organic solvent in comprehensive two-dimensional liquid chromatography (2DLC). It was shown that one can reach higher peak capacities only by using more eluent. The equilibration volume of the second dimension, however, did not affect the solvent consumption significantly. Calculations confirmed that the same target peak capacity could be achieved by consuming significantly different volume of organic modifier depending on the number of fractions analyzed in the second dimension suggesting that 2D separations can be optimized for eluent consumption. It was shown that minimization of eluent usage requires the use of small and high efficient columns in the second dimension. A simple equation was derived for the calculation of the optimal number of collected fractions from the first dimension that allowed the minimization of eluent usage, cost and environmental impact of comprehensive 2DLC separations.
开发了一种用于最小化全二维液相色谱（2DLC）中有机溶剂消耗的算法。结果表明，仅通过使用更多的洗脱液就可以达到更高的峰容量。然而，第二维的平衡体积对溶剂消耗的影响并不显著。计算结果证实，根据在第二维中分析的馏分数量，通过消耗显著不同体积的有机改性剂，可以达到相同的目标峰容量，这表明 2D 分离可以针对洗脱液消耗进行优化。结果表明，最小化洗脱液用量需要在第二维中使用小而高效的柱子。推导出了一个简单的方程，用于计算从第一维收集的最佳馏分数，从而可以最小化全二维 LC 分离的洗脱液用量、成本和环境影响。
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