Parsadanian H K, Ter-Tatevosian L P, Martikian H R, Avakian S H
Institute of Biochemistry, Armenian Academy of Sciences, Yerevan, USSR.
Mol Cell Biochem. 1989 Oct 31;90(2):185-90. doi: 10.1007/BF00221218.
Alloxan diabetes induced in white rats by intraperitoneal injection of alloxan-monohydrate (15 mg/100 g body weight) was used to study changes in the glycogen phosphorylase a and b, phosphoprotein phosphatases and hexokinase activities under insulin deficiency conditions. Among the enzymes studied, an increase in muscle phosphorylase a activity as well as the a/b ratio have been obtained. In diabetic muscle phosphoprotein phosphatases and hexokinase activities were diminished. AMP increased the liver glycogen phosphorylase activity twice in diabetic rats whereas in normal animals the enzyme was less sensitive to this effector. The changes in liver hexokinase activity at diabetes were not connected and correlated with the altered phosphorylase and protein phosphatase activities. The logical chain of probable molecular events taking place in muscle glycogen metabolism under the conditions of insulin deficiency is offered.
通过腹腔注射一水合四氧嘧啶（15毫克/100克体重）诱导的大白鼠四氧嘧啶糖尿病被用于研究胰岛素缺乏条件下糖原磷酸化酶a和b、磷蛋白磷酸酶以及己糖激酶活性的变化。在所研究的酶中，已观察到肌肉磷酸化酶a活性以及a/b比值增加。糖尿病肌肉中的磷蛋白磷酸酶和己糖激酶活性降低。在糖尿病大鼠中，AMP使肝脏糖原磷酸化酶活性增加了两倍，而在正常动物中该酶对这种效应物的敏感性较低。糖尿病时肝脏己糖激酶活性的变化与磷酸化酶和蛋白磷酸酶活性的改变没有关联且不相关。文中提出了胰岛素缺乏条件下肌肉糖原代谢中可能发生的分子事件的逻辑链。
