由Sfp磷酸泛酰巯基乙胺基转移酶催化的位点特异性蛋白质标记辅助的噬菌体筛选。
Phage selection assisted by Sfp phosphopantetheinyl transferase-catalyzed site-specific protein labeling.
作者信息
Zhao Bo, Zhang Keya, Bhuripanyo Karan, Wang Yiyang, Zhou Han, Zhang Mengnan, Yin Jun
机构信息
Department of Chemistry, University of Chicago, 929 E57th Street, Chicago, IL, 60637, USA.
出版信息
Methods Mol Biol. 2015;1266:161-70. doi: 10.1007/978-1-4939-2272-7_11.
Abstract
Phosphopantetheinyl transferases (PPTase) Sfp and AcpS catalyze a highly efficient reaction that conjugates chemical probes of diverse structures to proteins. PPTases have been widely used for site-specific protein labeling and live cell imaging of the target proteins. Here we describe the use of PPTase-catalyzed protein labeling in protein engineering by facilitating high-throughput phage selection.
摘要
磷酸泛酰巯基乙胺基转移酶（PPTase）Sfp和AcpS催化一种高效反应，该反应可将多种结构的化学探针与蛋白质缀合。PPTase已广泛用于靶蛋白的位点特异性蛋白质标记和活细胞成像。在此，我们描述了通过促进高通量噬菌体筛选，在蛋白质工程中使用PPTase催化的蛋白质标记。
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